Abstract The present disclosure relates to methods of measuring target nucleic acids by using coded molecules and analysis thereof by translocation through a nanopore. Generally, coded molecules pertain to a target polynucleotide dependent modification. The modified coded molecule is identified by isolating the modified coded molecules from the unmodified coded by testing a change in […]
Nowadays, the direct sequencing of PCR products has already played a significant role in molecular biology and genomics research. Such sequencing is widely applied to the detection of gene mutation, diagnosis of genetic diseases, and polymorphism research of single nucleotide. Compared with traditional clone sequencing, direct sequencing of PCR products conducts sequencing towards the amplified […]
Abbreviation for complementary deoxyribonucleic acid, cDNA is a form of DNA artificially synthesized from a messenger RNA template with the presence of appropriate primers. With the complementary single-stranded DNA chain, using the RNA as a template in the presence of appropriate primers, begins to synthetize. And after the synthesis of single cDNA, under the action of […]
With the breathtaking development of high-technology, an increasing number of biotechnological products or services have been born.And those products and services are being widely utilized in both science and medicine.Even some of them are applied in agriculture and farming business.Now, let’s have a look on Immune Repertoire Sequencing, a promising biotechnological service to amplify and […]
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