Based on PacBio Circular Consensus Sequences (CCSs), polymerase could copy the same DNA region for several times, which generates high-fidelity, long reads (>99.9% single-molecule read accuracy). Long Amplicon Analysis (LAA) using Single Molecule, Real-Time (SMRT) Sequencing and the Sequel System produces highly accurate and phased CCSs from long amplicons.
Figure 1. Overview of SMRT Sequencing Technology. (From Simon Ardui, et al., 2018)
Compared to the short-read sequencing platforms, PacBio's long read sequencing makes it is straightforward to sequence amplicons or captured fragments ranging in size from several hundred base pairs up to 10 Kb. These long sequences are very useful for visualization of variants including SNPs, CNVs, and other structural variants that typically do not require assembly.
CD Genomics provides professional and cost-effective LAA service with sequencing depth ranging from <500 to 10K CCSs per sample to meet your specific research requirements.
For full-length 16S/18S/ITS gene sequencing:
For other applications, please inquire.