Targeted RNA Sequencing

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Targeted RNA-sequencing selects and sequences subset transcripts of interests, increasing the coverage of a focused set of RNA sequences. It is an accurate method that generates genomic and gene-expression information of specific genome regions. The specific RNA sequences analysis enables RNA-Seq with high sensitivity and cost-effective access to NGS. By either targeted capture enrichment or amplicon-based approaches, Targeted RNA-Seq measures dozens to thousands of targets simultaneously. Enrichment assays also is a tool to identify both known and novel gene fusion partners in many sample types.

Providing qualitative and quantitative information for differential expression analysis, allele-specific expression measurement, and gene fusion verification, targeted RNA-sequencing can help to understand tumor classification and progression, genetic diseases and RNA drug response. Cancer transcriptome sequencing provides valuable information about gene expression changes in tumors. Profiling RNA-based drug response biomarkers aids too uncover multiple drug-susceptible tumorigenic pathways and improve the efficiency and success rate of the drug development process.

Advantages of Targeted RNA Sequencing

  • Provides a comprehensive analysis of the specific transcripts
  • Choose validated pathway, cell, or disease-specific panels
  • Compatible with low-quality, formalin-fixed, paraffin-embedded (FFPE)
  • Fast turnaround time and Highest data quality
  • Strand information on RNA transcripts
  • Effective transcriptome and pathways analysis 

Targeted RNA Sequencing workflow

We offer integrated targeted RNA-Seq workflows that simplify the entire process, from library preparation to data analysis and biological interpretation.

HLA Typing

Sample Requirements

  • Recommended concentration should be 20ug/uL or more.
  • A minimum of 2μg of the total is required.
  • A260: A280 value should be between 1.8 and 2.0
  • RNA should be stored in nuclease-free water or RNA Stable.
  • RNA must be validated using an assay such as a BioAnalyzer RNA chip. RIN value ≥ 7.0
  • Tissue, cell, blood, and blood products are acceptable.


  • Illumina High throughput sequencer
  • Flexible service options: single end or paired-end sequencing, optional reads number according to research goals.

Data analysis

  • Raw data QC
  • Alignment and TPM/RPKM/FPKM-based quantitation
  • Expression analysis
  • Statistics of SNPs/Indels
  • Alternative splicing analysis
  • GO and KEGG annotation
For Research Use Only. Not for use in diagnostic procedures.

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