The Introduction of Sanger Sequencing
Sanger sequencing, also known as the "chain termination method", is a method of DNA sequencing based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. Developed by two time Nobel Laureate Frederick Sanger and his colleagues in 1977, hence the name the Sanger Sequence, it was the most widely used sequencing method for approximately 40 years.
More recently, Sanger sequencing has been replaced by Next-Generation sequencing methods, especially for large-scale, automated genome analyses. However, Sanger remains useful for sequencing single genes or amplicon targets of up to 100 base pairs in length, for projects involving 96 or fewer samples, for microbial identification and gene fragment analysis, and for analyzing short tandem repeats. Moreover, Sanger is considered the “gold standard” sequencing method for validating the sequence of specific genes.
Sanger sequencing also is the most widely used testing platform for mutation detection in various cancer settings, as it provides a comprehensive examination of all genetic aberrations in the sample material. Sanger sequencing has proven useful for assessing the presence or absence of recurrent single nucleotide mutations or small insertions/deletions in oncogenes and tumor suppressor genes in surgically resected pathology specimens.
The Sanger (chain-termination) method for DNA sequencing
Key Features and Advantages
More than a decade of experience in Sanger sequencing makes CD Genomics the most trusted company for your DNA sequencing projects. Get high standard results with short delivery times and personalized customer support. Our highly-trained technical support provide you the best service on the market. If you have additional requirements or questions, please feel free to contact us.
Tuesday, May 30, 2023
Jorge Bouças, PhD
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