CD Genomics-the genomics service company
Genetic Linkage Map
Genetic Linkage Map
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Genetic Linkage Map

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Genetic linkage map, also known as genetic map, is a linear graph of the sequence and relative distance of molecular markers on chromosome based on the frequencies of recombination between markers during crossover of homologous chromosomes. The construction of high-density genetic map based on high-throughput sequencing technology has gradually become a revolutionary technology favored by researchers. It can rapidly develop a large number of molecular markers at one time, and obtain ultra-high-density genetic map. It provides accurate and complete QTLs number and locus information that co-segregate with the phenotype.

To address the emerging needs of research communities, CD Genomics has developed an affordable, reliable genetic linkage map service based on high-throughput sequencing to get dense markers and give researchers a high-quality genetic linkage map, as well as professional data analysis.

A genetic linkage map of Hawthorn.Figure 1. A genetic linkage map of Hawthorn (Yuhui Zhao, et al. 2020)

Applications of genetic linkage map

Applications of genetic linkage map

Genetic linkage map workflow

Genetic linkage map workflow

Service Specifications

Sample Requirements

  • Two parental lines, progeny of F1/F2≥150; progeny of RIL/DH≥100
  • DNA sample: ~1.5 μg (concentration ≥ 30 ng/μl; OD260/280=1.8~2.0)
Sequencing

  • Illumina HiSeq platforms
  • Parental lines 20-30X, progeny individual 3-5X
  • Analysis of sequencing quality metrics
Bioinformatics Analysis
We provide customized bioinformatics analysis including:

  • Raw data QC
  • Reference alignment
  • SNP mutation detection and annotation
  • Polymorphic marker development
  • Construction and evaluation of genetic linkage map

Delivery

  • Raw data(FASTQ)
  • Marker information
  • Genetic linkage map evaluation report
  • Data analysis report

Reference:

  1. Zhao YH, et al. High-density genetic linkage-map construction of hawthorn and QTL mapping for important fruit traits. PLoS ONE. 2020,15(2): e0229020.
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