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Chloroplast DNA (cpDNA) Sequencing

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The Introduction of cpDNA Sequencing

Chloroplasts are organelles found in plant cells and eukaryotic algae that conduct photosynthesis,they are not only essential for the life of plants but for all life on Earth. Chloroplasts, like mitochondria, contain their own DNA, comprising approximately 130 genes, which are involved in photosynthesis and other important metabolic processes. Chloroplast genomes display considerable variation both within and between species, providing insights into the phylogeny and evolutionary adaption. Chloroplasts also carry out a number of other functions, including fatty acid synthesis, much amino acid synthesis, and the immune response in plants.

Chloroplast DNA (cpDNA) Sequencing

The chloroplast DNA (cpDNA) sequencing is a high-throughput sequencing of plant chloroplast genomes using Illumina and PacBio platforms to perform an in-depth analysis of sequence information. Through comparative genomic analysis, information such as species classification, phylogenetic evolution, geographic lineage inheritance, disease diagnosis and forensic science is obtained, revealing its important role in species origin, biological evolution and genetic relationship between different species.

Key Features and Advantages

  • Flexible experimental protocol: adopt appropriate sequencing solutions according to different needs
  • Strong bioinformatics team: in addition to providing standard bioinformatics services, it also provides personalized bioinformatics services
  • Experienced: chloroplast sequencing of multiple species has been completed
  • Cost-effective and time-efficient

Project Workflow

Chloroplast DNA (cpDNA) Sequencing Workflow

Data Analysis

  • Data quality control
  • Clean Data assembly
  • Chloroplast genome annotation
  • Chloroplast genome map
  • Comparative genomic analysis
  • Phylogenetic analysis, species identification
  • More data mining upon your request
Sample Requirements

  • Illumina: DNA amount: ≥ 1 μg; DNA concentration ≥ 30 ng/μl,
  • PacBio: DNA amount: ≥ 15 μg; DNA concentration ≥ 50 ng/μl,
Sequencing Strategy

  • Illumina Hiseq PE150; At least 2G data per sample (sequence data is 0.5-1 times the nuclear genome)
  • PacBio Platform: Library size: 2K; At least 50X data per sample

CD Genomics offers cost-effective protocol of cpDNA sequencing, including DNA preparation, chloroplast enrichment, sequencing, and analyze the entire chloroplast genome from total DNA samples. We can tailor this pipeline to your research interest. If you have additional requirements or questions, please feel free to contact us.

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-275-3058
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Email: info@cd-genomics.com