HLA Typing

CD Genomics provides accurate and reliable HLA typing service based on next-generation sequencing (NGS) technology that generates unambiguous, phase-resolved HLA sequencing results in a single assay.

The Introduction to HLA Typing

The HLA (human leucocyte antigen) system encodes the major histocompatibility complex (MHC) proteins in humans. These integral cell membrane glycoproteins are responsible for the regulation of the human immune system. There are two classes of MHC, i.e., MHC class I and MHC class II. The HLA gene complex is located on a 3.6 Mb region within chromosome 6p21. They are the most polymorphic gene family found in the human genome, with more than 10,000 different HLA alleles reported to date, thus the capacity to mount an immune response can be dramatically different between individuals within a cohort selected from a single population. HLA genes have been strongly implicated in transplant rejection, autoimmune disease, vaccine pharmacogenomics, cancer, infectious diseases, and mate selection.

HLA genotyping is the identification of the HLA class I and class II gene polymorphisms for individuals, which is indispensable for transplant matching and disease association studies. Unambiguous HLA genotyping is technically challenging owing to high polymorphism in various genomic regions. The development of NGS has changed this landscape of genotyping. High-resolution HLA genotyping by using PCR and NGS is uniquely able to address limitations of traditional HLA genotyping and Sanger sequencing assays in patients. It enables robust, simple, high-quality, and high-throughput analysis of the key HLA genes, data can be phased to a minimum of 6 digits. Another advantage is that phasing problem is determined since DNA templates are derived from single molecules.

HLA Typing

Figure 1. The HLA region in Chromosome 6.

Our Advantages

  • High resolution and 100% accuracy
  • Flexible testing sites: such as HLA class I (A, B, C) and HLA class II (DQB1, DQA1, DPA1, DPB1, DRB1, DRB3, DRB4, DRB5)
  • Capture sequencing enabling a cost-effective, fast, and high-throughput manner
  • Strict quality control and rich practical experience
  • Sample-to-Report Solution
  • Our HLA typing service has been widely applied in organ transplantation, population evolution, gene therapy, as well as immunological disease and cancer studies.

HLA Typing Workflow

Our highly experienced expert team executes quality management following every procedure to ensure comprehensive and accurate results. Our HLA typing workflow is outlined below, including DNA isolation, HLA gene capture, library preparation, high-throughput sequencing, and bioinformatics analysis.

HLA Typing

Figure 2. Our workflow of HLA typing.

Service Specifications
Sample requirements and preparation
  • Samples types: whole blood, genomic DNA, Saliva, PBMC, and mouth swabs.
  • DNA amount ≥ 500 ng (concentration ≥ 20 ng/μl; OD260/280=1.8~2.0); 1-2 ml whole blood in EDTA anticoagulant tube; 2 swabs from each side of the cheek mucosa
  • The sample preparation protocol covers DNA isolation, purification, quantification, QC, etc.
Library preparation and sequencing
  • HLA region capture: HLA Class I, II, 11 loci
  • MiSeq platforms, paired-end 300 bp; PacBio's SMRT platform
Bioinformatics Analysis
  • Raw data QC
  • Alignment to IMGT/HLA database
  • Contig assembly
  • HLA typing and annotation
  • Correlation analysis (case/control)

The service is offered for R&D / non-diagnostic purposes only and results must not be utilized to inform patient management decisions. With our advanced sequencing platforms and years of industry experience, we guarantee you high-resolution data and comprehensive bioinformatics analysis. If you have additional requirements or questions, do not hesitate to contact us.

For Research Use Only. Not for use in diagnostic procedures.
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