CD Genomics offers SNP genotyping using the simple and affordable SNaPshot Multiplex System to allow efficient and quick results.
The Introduction of SNaPshot
The SNaPshot Multiplex System is a primer extension-based method for genotyping known SNP positions through the automated DNA analyzer invented by Applied Biosystems. Through its multiplexing capability, up to 10 SNPs can be analyzed in a single reaction by using unlabeled, user-defined primers. SNPs can be interrogated regardless of their position on the chromosome or separation from a neighboring SNP locus. The Multiplex Ready Reaction Mix helps ensure robust and reproducible analyses of multiplexed samples. SNaPshot Multiplex System can screen and confirm SNPs, and detect minor sequence variations.
Advantages of SNaPshot
Firstly, the primers are used to amplify the target SNPs fragment, and Exo I and the Shrimp Alkaline Phosphatase (SAP) are added to the amplification products to digest the primer and the remaining dNTPs. Then, the purified products are used as templates, and the PCR is performed using the sequencing enzyme, four fluorescent labeling ddNTP and the 5 ‘- terminal extension primers close to the SNP site. The primers extend one base only. After the ABI sequencer, the corresponding SNP loci are determined according to the position and color of the peak. According to the color of the peak, we can know the type of the base, and identify the gene sequence of the sample. It is usually used for analysis of 3 to 30 SNP sites. A typical SNaPshot Multiplex System for SNP genotyping workflow is shown as follow:
|Sample Requirements and Preparation
With professional bioinformatics capability, CD Genomics offers high-quality SNP genotyping services for you. If you have any questions, please feel free to contact us.