Complete Plasmid DNA Sequencing

CD Genomics provides complete plasmid DNA sequencing service, and our improved bioinformatics pipelines are available to perform de novo assembly with no reference required.

The Introduction of Complete Plasmid DNA Sequencing

Plasmids are perceived as mobile genetic elements that exist extra-chromosomally and occasionally carry accessory genes that confer an advantage to their host in its ecological niche. They are thus thought to play an important evolutionary role in microbial communities by laterally introducing genes and traits into microbial genomes. Plasmids are important vehicles for rapid adaptation of bacterial populations to changing environmental conditions. The genetic variation generated by plasmid carriage within populations ensures the robustness towards environmental change. Plasmid-mediated gene transfer plays an important role not only in the mobilization and dissemination of antibiotic resistance genes but also in the spread of degradative pathways and pathogenicity determinants of pathogens.

Despite their importance, technical obstacles still limit the plasmid study. Plasmid DNA sequencing is rapidly becoming a standard approach to increase our understanding of the genetic diversity and evolutionary history of plasmids. It not only helps to define the molecular events that took place during the evolution of these plasmids, but also give us a more complete overview of the enormous collection of accessory genes encoded on plasmids. And comparative plasmid sequence analysis has provided insights into the evolution of plasmids and their relatedness, their modular structure and the existence of hot spots for the insertion of accessory genes.

Key Features and Advantages

• Complete validation of known plasmids
• Full length sequencing of unknown plasmid
• Complete characterization of plasmid genomes
• No reference sequence required
• Accurate assembly
• Cost-effective high-throughput sequencing
• High quality data & fast turnaround

Project Workflow

Sequencing Strategy:

Illumina Platform, Library size: 400-500 bp, PE 250, 100X
PacBio Platform: Library size: 4 K, 100X

Data analysis

• Data quality control
• High quality data acquisition
• Genome de novo assembly
• Detection of plasmid-specific sequences
• …and more

Analysis pipeline

CD Genomics’s plasmid sequencing service utilizes the power of Illumina Platform for plasmid verification, and has developed a low-cost high-throughput approach for complete larger plasmid sequencing using PacBio SMRT technology. We are pleased to use our extensive experience and advanced platform to offer the best service and the most qualified products to satisfy each demand from our customers.