Whole Genome Re-Sequencing Service

What Is Whole Genome Re-Sequencing

Whole genome re-sequencing is the process of sequencing the genomes of different individuals within a reference genome species, followed by differential analysis of individuals or populations based on the obtained data. Genome resequencing is primarily used to assist researchers in identifying various types of variations such as single nucleotide polymorphisms (SNPs), copy number variations (CNVs), and insertions/deletions (Indels), thus expanding the genetic characteristics of a biological population from a single reference genome at a lower cost. Whole-genome resequencing is widely applied in the study of human diseases and animal and plant breeding, including the detection of pathogenic genes, genetic variations, prenatal screening, personalized medicine, and more.

Whole Genome Re-Sequencing Service Offering

Sequencing Extraction of genomic DNA, fragmentation, gel recovery of desired DNA fragments (0.2~0.5Kb), adapter ligation, cluster preparation, and ultimately resequencing of the inserted fragments using the Paired-End (Solexa) method.

Data Processing After sequencing, the obtained reads are processed to filter out adapter contamination and other pollutants. The processed reads are then aligned to a reference genome for quality assessment of sequencing depth, coverage, uniformity, and other output data.

Advantages of Whole-Genome Resequencing

Provides a high-resolution genomic base sequence map.

Enables feature analysis of any genome by combining short insertions and longer fragments.

Identifies disease-causing alleles that may not have been identified through other methods.

Identifies potential pathogenic variations, providing information for further in-depth research on gene expression and regulatory mechanisms.

Our Service Advantages

Accelerated Resequencing Analysis

Customized Analysis Strategies: Based on different sequencing species and protocols, we offer customized options such as selecting reference genome versions, alignment algorithms, and annotation databases.

Comprehensive Database Integration: We continuously update and integrate multiple databases and versions to provide accurate gene information and annotations.

Powerful Omics Integration Analysis Capability: By integrating genome resequencing with other technologies such as transcriptome sequencing and methylation sequencing, we expand beyond single-gene variation data.

Workflow of Whole-Genome Resequencing

Related Solutions

Cancer Whole-Genome Sequencing: Sequencing of the entire genome of tumors and corresponding normal tissues provides comprehensive insights into the specific mutations associated with cancer. It facilitates the study of oncogenes, tumor suppressor genes, and other risk factors.

Plant and Animal Sequencing: Whole-genome sequencing of plants and animals is an effective method to explore genes, SNPs, and structural variations while determining the genotype. This information can provide existing gene maps and improve agricultural breeding and screening processes.

Pathogenic Variation Investigation: Whole-genome sequencing of the human genome enables the identification of disease-related single nucleotide variations and copy number variations. By identifying these pathogenic variations, in-depth genetic research can be conducted targeting pathological features.