CD Genomics Blog

Two induced pluripotent stem cell (iPSC) lines can look equally healthy, express OCT4 and NANOG, pass a routine pluripotency panel, and still produce strikingly different organoids. One line generates organized, target-rich structures across repeated batches. The other forms irregular aggregates, drifts toward unintended lineages, or never reaches the developmental checkpoint required for a useful model. […]

Pooled barcoding has changed the scale of lipid nanoparticle (LNP) discovery. Instead of administering one formulation to one group, investigators can associate each formulation with a unique sequence, combine the formulations, and use sequencing to compare their recovery. The approach saves experimental capacity, but it also compresses several biological events into a deceptively simple output: […]

A compact RNA-guided nuclease can fit an attractive delivery design and still edit poorly in the cell type that matters to a research project. The obvious response is to modify its protein sequence. The difficult question is which variants to test when the number of possible amino-acid substitutions is enormous and the number of reliable […]

Primary human monocytes, macrophages, and dendritic cells are often the cells investigators most want to perturb and the cells least forgiving of a standard pooled CRISPR workflow. They are finite, donor variable, difficult to expand, sensitive to delivery stress, and ready to alter their state when exposed to nucleic acid or particle preparations. A pooled […]

A prime-editing model can return a clean, confident score for every candidate pegRNA. That score is useful, but it is not an experimentally measured editing rate and it is not a pass/fail decision. The gap matters because prime editing is a sequence-programmed reaction that is completed by a living cell. A candidate can look favorable […]