What is the 16S rRNA 16S rRNA (16S ribosomal RNA), is a component of the prokaryotic ribosome 30S subunit. The “S” in 16S is a sedimentation coefficient, that is, an index reflecting the downward velocity of the macromolecule in the centrifugal field. The higher the value, the greater the molecule. The 16S rRNA gene is […]
Microsatellites, also known as simple tandem repeats (STRs), are simple repeat sequences distributing in eukaryotic genomes, which are composed of 2 ~ 6 nucleotide tandem repeats. The repeating times in individual are variable, therefore, the application of microsatellite markers is very extensive. Microsatellite loci is usually amplified by PCR. PCR products are analyzed by electrophoresis […]
Summary: a DNA methylation age inference method, combined with Sequenom MassARRAY technology, can reduce the error of the age to 4.7 years. Age estimation is one of the most important topics in the field of forensic medicine. Especially for juvenile delinquency, highly corrupt bones of the dead and nameless corpse, it can provide the scope […]
Genome-wide association study (GWAS) is a kind of method to detect all or most of the genes among different individuals in specific species and to understand the genetic variation between different individuals. Many susceptibility genes and loci have been found in colorectal cancer using the GWAS method, but there are still many unknown susceptibility genes […]
Jun 7, 2016, single cell transcription and methylation analysis have become the powerful tools for single cell research. However, it is difficult to reveal the direct correlation between DNA methylation and gene expression in single cells. This is because there is a large difference between the cells, and we can not detect a cell’s transcription […]
Whole exome sequencing (WES) is now being used more and more in the field of research and diagnosis. Researchers are expected to study the entire coding regions of known genes with adequate sequencing depth and coverage. Currently a large number of exome capture platforms on the market have there respective advantages and some differences in […]
Like many researchers to do hundreds of pieces of Southern blots, I first used PCR genotyping with soon efficiency. It no longer needed such as a few days to see the results and no longer needed DNA microscopic images or UV. With the continuous progress of technology, RCR makes the genomics and transcriptomics change dramatically, […]
A common tumor suppressor gene was found in the patients with acute leukemia (AL). The finding reveals its synergistic effect between function abnormalities and a variety of different carcinogenic gene, providing an important foundation for research and development of novel leukemia treatment. Researchers found the transfer functional MLL – NRIP3 carcinogenic gene and SETD2 genetic […]