CD Genomics Blog

Explore the blog we’ve developed, including genomic education, genomic technologies, genomic advances, and genomics news & views.

The NextSeq Sequencing Systems from Illumina is a powerful and versatile system for high-throughput sequencing applications. It’s designed to deliver rapid and accurate results across various sequencing workflows, making it a popular choice for research, clinical, and applied genomics projects. This series is available in a variety of models Illumina NextSeq500, NextSeq550, NextSeq1000 and NextSeq 2000 sequencing systems.

At CD Genomics, we utilize Illumina, MGI, PacBio SMRT and Oxford Nanopore sequencing platforms for our sequencing services. CD Genomics also offers its expertise in SNP and microsatellite genotyping on a wide range of platforms.

Please refer to our Sequencing Platform for more information.

NextSeq 500 vs. NextSeq 1000

Table 1 NextSeq 500 vs. NextSeq 1000

Systems NextSeq 1000 NextSeq 550
Chip Scanning Capability None – CytoSNP-850K Beadchip
– CytoSNP-12 BeadChip
– HumanKaryomap-12 BeadChip
Light Source Laser wavelengths of 449 nm, 523 nm, 820 nm LED wavelengths of 520 nm, 650 nm
Instrument Dimensions 60 cm (Width) x 65 cm (Depth) x 60 cm (Height) Weight: 141 kg 53.3 cm (Width) x 63.5 cm (Depth) x 58.4 cm (Height) Weight: 83 kg
Operating Modes – 2x50bp/40Gb
– 2x100bp/80Gb
– 2x150bp/120Gb
High-throughput flow cell:
– 1x75bp/30Gb
– 2x75bp/60Gb
– 2x150bp/120Gb
Mid-throughput flow cell:
– 2x75bp/19Gb
– 2x150bp/39Gb
Data Output & Quality – 40Gb, Q30>85%, 13h
– 120Gb, Q30>75%, 29h
– 16Gb, Q30>80%, 15h
– 120Gb, Q30>75%, 29h

How to Choose Between NextSeq 1000 and NextSeq 550?

If you need lower startup throughput, chip scanning capability, or have in vitro diagnostic (IVD) requirements, then choose NextSeq 550. If you prefer lower sequencing costs, faster analysis speed, or intend to conduct single-cell sequencing analysis, then choose NextSeq 1000.

NextSeq 2000 Sequencing System

The NextSeq 2000 is exceptionally well-equipped to address future demands, offering robust sequencing capabilities for high-throughput applications.

Table 2 NextSeq 2000 Sequencing System

Feature Description
System Type All-in-one benchtop sequencer
Sequencing Reagents 10 different sequencing reagents available
Maximum Throughput 360 Gb in a single run
Maximum Read Length 600 cycles
Applications Supported – 16S metagenome sequencing
– Exome sequencing
– Disease analysis
Fast Genome Analysis Server On-board server for accurate and fast genome analysis
Data Compression Lossless compression reduces data storage space by up to 80%
Integrated Reagent Cartridges Simplifies operation with integrated reagent cartridges
Modular Design Features a no-clean dry system for fast and environmentally friendly operation

Applications of NextSeq 2000

  • Single-cell Gene Expression Analysis

Uncover the transcriptome of individual cells, providing a nuanced understanding of cell-to-cell variability. Explore diverse biological properties within complex tissues and decipher how different cell subpopulations respond to environmental cues. With P2 reagent, analyze up to 10 samples in approximately 13 hours, or utilize P3 reagent to process 25 samples in about 19 hours.

  • Whole Exome Sequencing

Offering a cost-effective alternative to whole-genome sequencing, exome sequencing efficiently identifies coding variants essential for various applications such as population genetics, genetic diseases, and cancer research. With P2 reagent, analyze 16 samples in approximately 21 hours, or utilize P3 reagent to obtain 40 complete exomes in about 33 hours.

Comprehensive detection of genes across all organisms within complex samples. Assess bacterial diversity, determine microbial abundance in diverse environments, or study unculturable microorganisms beyond the reach of conventional methods. Process data from 8 samples in approximately 29 hours with P2 reagent, or scale up analysis with P3 reagent to handle data from up to 20 samples in about 48 hours.

Related Services

Whole Genome Sequencing
Whole Exome Sequencing
Metagenomic Shotgun Sequencing

Leave a Reply

Your email address will not be published.