CD Genomics Blog

Explore the blog we’ve developed, including genomic education, genomic technologies, genomic advances, and genomics news & views.

Blog Archives

DNA Sequencing: Definition, Methods, and Applications

What Is DNA Sequencing? DNA Sequencing is the method that determines the order of the four nucleotides bases (adenine, thymine, cytosine, and guanine) that make up the DNA molecule and convey important genetic information. In the DNA double helix, the four bases bond with the specific partner to form units called base pairs (bp). Adenine […]

Sanger Sequencing: Introduction, Principle, and Protocol

What Is Sanger Sequencing? Sanger sequencing, also known as the “chain termination method,” was developed by the English biochemist Frederick Sanger and his colleagues in 1977. This method is designed for determining the sequence of nucleotide bases in a piece of DNA (commonly less than 1,000 bp in length). Sanger sequencing with 99.99% base accuracy […]

Chloroplast Fact Sheet: Definition, Structure, Genome, and Function

Chloroplast Definition Chloroplast, found in plant cells and some protists such as algae and cyanobacteria, is a cell organelle known as a plastid. Chloroplasts are the food producers of the cell by converting light energy of the sun into sugar that can be used by the cell. This entire process is known as photosynthesis and […]

Mitochondria Fact Sheet: Definition, Structure, Function and Genome

What Are Mitochondria? Mitochondria are recognized as the powerhouse of the cell since they help convert oxygen and nutrients into adenosine triphosphate (ATP), the energy currency of the cell. Mitochondria are small organelles (usually 0.75~3 micrometers), found in nearly all types of human cells except mature red blood cells, as well as cells of other […]

Introduction to DNA Microarray Technology

What is a DNA Microarray? Microarray is a common laboratory tool for detecting gene expression or gene mutations in a high throughput manner. These slides are also known as gene chips or DNA chips. Thousands of probes (with known identity) are immobilized on a microscope slides or silicon chips or nylon membrane, with thousands of […]

Introduction to Next Generation Sequencing Technology

Next generation sequencing (NGS), high-throughput sequencing, or massively parallel sequencing are related terms that describe the DNA sequencing technology which has revolutionized the biological research. Characterized by ultra-high throughput, scalability, and speed, NGS enables researchers to sequence a human genome within a single day, which Sanger sequencing took over a decade to accomplish the final […]

Principle and Workflow of Illumina Next-generation Sequencing

Illumina, established in 1998 in San Diego, CA, is a leading company in the field of sequencing. In 2006, Illumina acquired Solexa, got the next-generation high-throughput sequencing technology and developed it into a mainstream technology on the market. It currently provides sequencing systems such as MiSeq, HiSeq 2500, HiSeq 3000, HiSeq 4000, HiSeq X Ten, HiSeq […]

Basic Facts about Microsatellite Genotyping—Introduction to Microsatellites

Microsatellites, also known as simple tandem repeats (STRs), are simple repeat sequences distributing in eukaryotic genomes, which are composed of 2 ~ 6 nucleotide tandem repeats. The repeating times in individual are variable, therefore, the application of microsatellite markers is very extensive. Microsatellite loci is usually amplified by PCR. PCR products are analyzed by electrophoresis […]

Genotyping by PCR

Like many researchers to do hundreds of pieces of Southern blots, I first used PCR genotyping with soon efficiency. It no longer needed such as a few days to see the results and no longer needed DNA microscopic images or UV. With the continuous progress of technology, RCR makes the genomics and transcriptomics change dramatically, […]

What Do You Know About the Pacbio RS System

The PacBio RS system is the third generation sequencing platform based on the SMRT sequencing system which is invented by Pacific Biosciences company. It can do the job of sample preparation, sequencing and sequencing read in one day. It has some advantages that the second generation sequencing technology doesn’t have: splicing of a large amount […]