Monthly Archives: February 2020

Sanger Sequencing: Introduction, Principle, and Protocol

Sanger Sequencing: Introduction, Principle, and Protocol

What Is Sanger Sequencing? Sanger sequencing, also known as the “chain termination method,” was developed by the English biochemist Frederick Sanger and his colleagues in 1977. This method is designed for determining the sequence of nucleotide bases in a piece of DNA (commonly less than 1,000 bp in length). Sanger sequencing with 99.99% base accuracy […]

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