Pyrophosphate sequencing represents an innovative enzymatic cascade chemiluminescence sequencing method driven by four key enzymes: DNA polymerase, ATP sulfurylase, luciferase, and adenosine triphosphate bisphosphatase (apyrase). This cutting-edge technology enables real-time detection of biological light signals emitted during DNA synthesis, setting a groundbreaking precedent for simultaneous sequencing and synthesis. In the realm of early tumor screening […]

What is Exon? A coding sequence within a fragmented gene, an exon (expressed region) constitutes a vital segment of a eukaryotic gene. It remains conserved post-splicing and serves as the basis for protein expression during protein biosynthesis. These nucleotide sequences are present in both the initial transcription product and the mature RNA molecule. Genome encompasses […]

The study of population evolution relies on advanced genomic techniques like whole-genome resequencing or other sequencing methods. These technologies enable the acquisition of genomic data from diverse subpopulations within a species. By extracting extensive variant information such as single nucleotide polymorphisms (SNP), insertions/deletions (InDel), structural variations (SV), and copy number variations (CNV), researchers delve into […]