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Illumina Next-Generation Sequencing: Principles, Workflow, and Optimization Logic

Illumina Next-Generation Sequencing: Principles, Workflow, and Optimization Logic

Illumina sequencing by synthesis (SBS) has become the dominant platform in genomics not because it is simple, but because its underlying engineering—from surface chemistry to optical detection—has been refined through two decades of continuous innovation. Understanding what happens inside the sequencing instrument is essential for designing robust experiments, troubleshooting failed runs, and interpreting data quality […]

Introduction to Next Generation Sequencing Technology

Introduction to Next Generation Sequencing Technology

Next generation sequencing (NGS), high-throughput sequencing, or massively parallel sequencing are related terms that describe the DNA sequencing technology which has revolutionized the biological research. Characterized by ultra-high throughput, scalability, and speed, NGS enables researchers to sequence a human genome within a single day, which Sanger sequencing took over a decade to accomplish the final […]

Improvement of Nanopore Sequencing: The Accuracy Was Greatly Improved

Improvement of Nanopore Sequencing: The Accuracy Was Greatly Improved

As a new technology of sequencing, the biggest advantage of nanopore is its low cost. Compared to other sequencing technology, such as sanger sequencing and next generation sequencing, the nanopore sequencing does not need any fluorescein for purification, and the process of DNA amplification is also unnecessary. It can save both money and time. Besides, […]

GEN Prediction: Next-generation Sequencing Market Will Reach $4.9 Billion in 2020

GEN Prediction: Next-generation Sequencing Market Will Reach $4.9 Billion in 2020

Sequencing technology has experienced a very long time, the next generation sequencing (NGS) radically change the way the human genomes are sequenced.IQ4I Research & Consultancy analysis suggests that global NGS market is expected to reach $4.892 billion by 2020 at a CAGR (a compound annual growth rate) of 20.7%. Among the global NGS market, instruments […]

The World of Transcriptomics Sequencing

The World of Transcriptomics Sequencing

The transcriptome is the set of all RNA molecules, including mRNA, rRNA, tRNA, as well as other non-coding RNA produced in one or a population of cells. It is different from the exome because it includes only those RNA molecules found in a specified cell population, and usually includes the amount or concentration of each […]

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