CD Genomics Blog

Explore the blog we’ve developed, including genomic education, genomic technologies, genomic advances, and genomics news & views.

Yearly Archives: 2022

How Does PacBio SMRT Sequencing facilitate AAV Vector Development?

Recombinant adeno-associated virus (AAV) has emerged as one of the primary vectors for gene therapy. This is because it has many desirable properties, including lack of pathogenicity, efficient infection of dividing and non-dividing cells, and sustained maintenance of the viral genome. With an excellent safety profile in numerous preclinical studies and hundreds of clinical trials […]

How to Sequence Chloroplast Genome and Why?

Introduction As one of the organelles that contain their own genome, chloroplasts (cp) are plastids with double membranes and vesicle-like structures. These tiny organelles convert solar energy, synthesize organic matters, and feed almost the entire life on Earth while providing for their own needs. However, it seems that chloroplasts are not satisfied with this, as […]

Genome Sequencing Drives Progress in Mitochondrial Diseases Research

Mitochondrial diseases (MitD) are an important clinical or medical challenge. Research has shown that genome sequencing can help to enhance the understanding of the genetic causes of MitD and to accelerate the development of functional research, clinical tests and therapeutic tools. What is Mitochondrial Disease? Mitochondria are membrane-bound organelles found in most eukaryotic cells that […]

RNA Sequencing for The Analysis of Exosome Transcriptome

Introduction to Exosomes Exosomes are small extracellular vesicles (EVs), ranging from 30 to 100 nm in diameter, carrying heterogeneous cargo, including miRNA, mRNA, lncRNA, lipids, and proteins. Exosomes widely present in very large quantities in all eukaryotic cells, mediating intercellular communication after being released into the extracellular space. In humans, exosomes nearly exist in all […]

What Is Ribo-Seq (Ribosome Footprinting)?

Introduction to Ribosome Profiling Ribosome-profiling techniques were developed in response to the importance of measuring gene expression at the translational level. Ribosome profiling is a direct technique for determining the precise mRNA region read by the ribosome and for directly examining the transcripts associated with the translation machinery. Ribosome profiling is a cellular snapshot of […]

Utilizing ATAC-Sequencing Approach to Predict Transcription Factors Binding in Individual Cells

Introduction to Transcription Factors Molecules important in controlling gene expression are transcription factors. Normally they are proteins, although they are composed of short, non-coding RNA as well. Transcription variables are also typically seen working in groups or complexes, creating multiple connections that allow varying degrees of control over transcription rates. Transcription factors (TFs) attach to […]

How to Analyze Single-Cell Genomics Data?

Introduction to Single Cell Genomics James Eberwine et al. and Iscove and colleagues were the first to sequence an entire transcriptome at the level of a single cell, using linear amplification by in vitro transcription and exponential amplification by PCR, respectively, to expand the complementary DNAs (cDNAs) of a single cell. These methods were first […]