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Microbial Diversity Analysis – 16S/18S/ITS Sequencing

Microbial Diversity Analysis – 16S/18S/ITS Sequencing

Our microbial diversity analysis platform based on 16S/18S/ITS amplicon sequencing aims to characterize microbial diversity efficiently and economically by utilizing the next/third generation sequencing technology and other molecular technologies. Our scientists utilize this platform to assist our customers for meeting both scientific and regulatory requirements.

Our Advantages:

Targeted region sequencing, enabling a rapid and efficient workflow.
Our team consists of more than 70% PhD and Masters-level scientists.
Extensive experience.
Advanced sequencing platforms. Both Illumina PE250/300 and PacBio systems are available to satisfy diverse sequencing requirements.

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We are dedicated to providing outstanding customer service and being reachable at all times.

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Introduction to Our Microbial Diversity Analysis Platform

Our integrated and novel microbial diversity detection platform delivers high-throughput, and accurate 16S/18S/ITS analysis via a combination of advanced sequencing technology and quantitative PCR (q-PCR). This platform allows both short-read 16S/18S/ITS sequencing by next-generation sequencing technology and full-length 16S/18S/ITS sequencing by PacBio SMRT sequencing or Nanopore sequencing. The results are validated by Sanger sequencing or q-PCR with genus- and species-specific primers. Quantitative PCR can also be used to quantify microbial species.

Our microbial diversity analysis platform can be used for both prokaryotic diversity analysis (including 16S rRNA analysis of bacteria, archaea, and actinomycetes) and eukaryotic diversity analysis (including fungal ITS sequencing and eukaryotic 18S rRNA sequencing) based on the role of 16S/18S/ITS as the molecular marker in prokaryotes or eukaryotes.

Microbial Diversity Analysis Workflow

Bioinformatics Analysis

Our bioinformatics analysis includes five parts: species annotation, diversity analysis, evolutionary analysis, functional profiling, and correlation analysis. For advanced bioinformatics analysis in detail, please refer to the following table.

Bioinformatics analysis		The problem to be solved
Species annotation		OTU clustering, species annotation
Diversity analysis	α diversity	Species richness within a single microbial ecosystem
	β diversity	Diversity in microbial community between different environments
	Meta-analysis	Comparative studies of abundant features between datasets
Evolutionary analysis		Construction of phylogenetic tree
Functional analysis	Analysis of the Intestinal Microflora	Locate the gut bacteria, predict human disease, and provide disease treatment
	PICRUSt	Predict functional profiles
	FAPROTAX	Predict the process of biochemical cycles
	Tax4fun	Predict functional profiles
	FunGuild	Predict the ecological roles of eukaryotes
Correlation analysis	CCA	Study the relationships between microbes and environmental factors
	VPA
	Network analysis

Sample Requirement

OD_260/230 ≥ 1.8, 1.8 < OD_260/280 < 2.0, no degradation or contamination.
Illumina platform: DNA amount ≥ 300 ng, PCR Products ≥ 400 ng
PacBio platform: gDNA ≥ 100 ng, PCR Products ≥ 400 ng

Deliverables: raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control dashboard, q-PCR data, statistic data, and your designated bioinformatics report.

* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.