Intestinal microbiome is a complex microbial community coexisted in the intestine of animals, and they are like an organ with various physiological functions such as metabolism, immunity and endocrine. Beneficial microorganisms are essential for proper bowel function, for example, helping to absorb water, synthesize vitamins, eliminate harmful substances and avoid the invasion of pathogenic bacteria. On the contrary, excessive harmful microorganisms will lead to the invasion of pathogens, changes in intestinal peristalsis, production of harmful substances and poor excretion. Several studies pointed out that intestinal microbial disorders are associated with diseases such as obesity, cardiovascular disease, diabetes and so on.Request a Quote
Based on our next-generation sequencing (NGS), PacBio SMRT sequencing, and Nanopore sequencing platforms, we have tested and analyzed various intestinal microbiome samples. We can help you identify the microorganisms presented in the gut, their relative abundance, and evolutionary relationships. We also study the correlation between intestinal microbes and human health, the function of intestinal microorganisms from gene level can also be studied, all of which are of great importance for scientific research, clinical use, and the development of supplements and therapies.
Note: Our services are for research use only, not for disease diagnosis and treatment.
Human and animal feces or intestinal contents. Our successful cases involve gut microbiome samples from human, mouse, rat, monkey, chicken, sheep, fish, horse, donkey, panda, pangolin and other animals.
Bacteria, fungi, actinomycetes, etc.
We are equipped with Illumina Hiseq/Miseq, Ion PGM, PacBio SMRT systems, Nanopore systems, PCR-DGGE (PCR-denaturing gradient gel electrophoresis), real-time qPCR, Clone library, and other detection platforms.
DNA sample: total DNA ≥ 300 ng, concentration ≥ 10 ng/μL, OD260/280=1.8-2.0
Be ensure that the DNA is intact and non-degradative, avoiding repeated freezing and thawing cycles.
Please use enough dry ice or ice packs during shipment.
We provide a broad range of oral and fecal sample collection kits for microbial recovery and analysis.
Figure 1. High-throughput sequencing analysis process
Figure 2. PCR-DGGE analysis process
|Basic Analysis||Routine Analysis (According to Customer Requirements)||Advanced Data Analysis|
|Sequence Filtering and Trimming||Heatmap||Phylogenetic Tree|
|Sequence Length Distribution||VENN||LDA-Effect Size (LEfSe)|
|OTU Clustering and Species Annotation||Principal Components Analysis (PCA)||Network Analysis|
|Diversity Index||Microbial Community Structure Analysis||Correlation Analysis|
|Shannon-Wiener Curve||α Diversity Index Analysis|
|Rank-Abundance Curve||Matastats Analysis|
|Rarefraction Curve||Weighted Unifrac test|
|Multiple Contrast||CCA/RDA Analysis|
|Principal Components Analysis (PCA)|