Gut Microbiome Research Solution

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MicroCollect™ Stool Collection and Preservation Device

The Importance of Gut Microbiome

Intestinal microbiome is a complex microbial community coexisted in the intestine of animals, and they are like an organ with various physiological functions such as metabolism, immunity and endocrine. Beneficial microorganisms are essential for proper bowel function, for example, helping to absorb water, synthesize vitamins, eliminate harmful substances and avoid the invasion of pathogenic bacteria. On the contrary, excessive harmful microorganisms will lead to the invasion of pathogens, changes in intestinal peristalsis, production of harmful substances and poor excretion. Several studies pointed out that intestinal microbial disorders are associated with diseases such as obesity, cardiovascular disease, diabetes and so on.

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Accelerate Research and Practice in Gut Microbiome

Based on our next-generation sequencing (NGS), PacBio SMRT sequencing, and Nanopore sequencing platforms, we have tested and analyzed various intestinal microbiome samples. We can help you identify the microorganisms presented in the gut, their relative abundance, and evolutionary relationships. We also study the correlation between intestinal microbes and human health, the function of intestinal microorganisms from gene level can also be studied, all of which are of great importance for scientific research, the development of supplements and therapies.

The Main Research Direction of Gut Microbiome

  • Exploring the differences in microbial composition and content between the disease group and the healthy group to search for disease-related markers
  • Studying and comparing the differences of intestinal flora structure between individuals at different intervention stages
  • Studying changes in intestinal flora of same individual at different developmental stages
  • Studying the changes in intestinal flora during the development of a certain disease, so as to search for biomarkers

What Can We Do?

  1. 1. Identification of intestinal microorganisms
  2. 2. Quantitation of intestinal microorganisms
  3. 3. Studying the evolution and function of microorganisms, and the relationship between microorganisms and diseases

Note: Our services are for research use only, not for disease diagnosis and treatment.

MicroCollect™ Sterile Pack Swabs

Detectable Objects

Human and animal feces or intestinal contents. Our successful cases involve gut microbiome samples from human, mouse, rat, monkey, chicken, sheep, fish, horse, donkey, panda, pangolin and other animals.

Detectable Microorganisms

Bacteria, fungi, actinomycetes, etc.

Technical Platforms

We are equipped with Illumina Hiseq/Miseq, Ion PGM, PacBio SMRT systems, Nanopore systems, PCR-DGGE (PCR-denaturing gradient gel electrophoresis), real-time qPCR, Clone library, and other detection platforms.

Sample Requirement

  • DNA sample: total DNA ≥ 300 ng, concentration ≥ 10 ng/μL, OD260/280=1.8-2.0
    Be ensure that the DNA is intact and non-degradative, avoiding repeated freezing and thawing cycles.
    Please use enough dry ice or ice packs during shipment.
    We provide a broad range of oral and fecal sample collection kits for microbial recovery and analysis.


Figure 1. High-throughput sequencing analysis process

PCR-DGGE analysis processFigure 2. PCR-DGGE analysis process

Bioinformatics Analysis

Basic Analysis Routine Analysis (According to Customer Requirements) Advanced Data Analysis
Sequence Filtering and Trimming Heatmap Phylogenetic Tree
Sequence Length Distribution VENN LDA-Effect Size (LEfSe)
OTU Clustering and Species Annotation Principal Components Analysis (PCA) Network Analysis
Diversity Index Microbial Community Structure Analysis Correlation Analysis
Shannon-Wiener Curve α Diversity Index Analysis
Rank-Abundance Curve Matastats Analysis
Rarefraction Curve Weighted Unifrac test
Multiple Contrast CCA/RDA Analysis
Principal Components Analysis (PCA)
* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.
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