Phosphorothioation (PT) is a post-replicative alteration of the DNA backbone in which a sulfur atom is substituted for the non-bridging oxygen in the phosphate substituents of the DNA sugar-phosphate backbone. Since PT-modified oligonucleotides are resilient to nuclease deterioration, and because the sulfur atom imparts many beneficial chemical properties, different molecular biological techniques have exploited this alteration.PT alteration genes have been defined in phylogenetically diversified bacteria and archaea supporting the exploration of microbial epigenetics.
PT-linked dinucleotides are created by enzymatic DNA hydrolysis in relation to canonical mononucleotides because of their nuclease resistance, facilitating the identification of PT alterations by LC-MS/MS. Additionally, to plot genomic PT areas, single-molecule, SMRT sequencing technology must be implemented. PacBio SMRT sequencing is being provided by CD Genomics to supplement our NGS service. We are delighted to give innovative genome de novo assembly alternatives and full-length gene/transcript sequencing strategies to meet your project requirements by taking full advantage of the long-read and single molecular sequencing potential established by PacBio.
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Single Molecular Real-Time (SMRT) sequencing uses an advanced flow cell with many hundreds of transparent bottoms of individual picolitre wells, zero-mode waveguides (ZMW). The polymerase is placed at the bottom of the well and enables the progression of the DNA strand through the ZMW. As a result, the structure can concentrate on a single molecular element. SMRT sequencing enables fluorescently tagged nucleotides that are modulated along with individual DNA layout molecules to be imaged in real-time. When the template and polymerase detach, the sequencing reaction terminates. The PacBio instrument's average read length is estimated at 2 kb, and some reads may exceed 20 kb. For de novo arrays of novel genomes that can stretch many more repeats and bases, longer reads are particularly useful.
Highly repetitive elements discovered in both eukaryotic and prokaryotic genomes present a challenge to the assembly of genomes and make it difficult to thoroughly analyze repetitive sequences. Long-read sequencing provides reads exceeding several or tens of kilobases (kbs) that can spread complicated or repetitive regions with a single continuous reading, enabling these large structural features to be resolved. It can also show where methylated bases take place, in addition to significantly longer and extremely reliable DNA sequences from individual unamplified molecules, offering functional data about genome-encoded DNA methyltransferases. In research of de novo genomics, metagenomics, transcriptomics, and epigenetics, PacBio SMRT sequencing has distinct benefits.
Our bioinformatics analysis includes five parts: Taxonomy Annotation, Gene Prediction, KEGG, eggNOG, and CAZy analyses. Our bioinformatics analysis services are flexible to your specific projects.
|Gene Prediction||Gene localization along a genome|
|KEGG||Recognizing high-level biological system processes and utilities|
|eggNOG||Set up relationships in orthology among genes|
|CAZy||Evaluation of Carbohydrate-Active Enzyme genomic, structural and biochemical details|
|Taxonomy Annotation||In a reference dataset, define the taxonomy of a sequence|
Sampling kits: We provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.