Microorganisms present in the air include bacteria, virus, and fungi. These biological particles can contribute to the ecological balance and play an important role in agriculture, the biosphere, cloud formation, global climate, and atmospheric dynamics. However, they also affect the environmental equality, and even provide a medium for the spread of air pollution and human diseases. Airborne microorganisms are a major cause of human respiratory diseases, such as asthma, allergies, and pathogenic infections. Both cultivation-dependent and –independent methods can be used to explore microbial diversity in the air and then uncover their impacts on the natural environment and human disease.Request a Quote
We analyze the microbiome of various environments including indoor and outdoor air, and their impact on human health using sequencing techniques like metagenomics sequencing, 16S/18S/ITS sequencing, and metranscriptomic sequencing. RT-qPCR is also used for achieving the qualitative and quantitative analysis of DNA or RNA. Bioinformatics analysis is required to process raw reads and determine the taxonomic classifications, biological functions for ecological environment, and effects on human health.
Note: Our service is for research use only, and not for therapeutic or diagnostic use.
The various microbiome samples from indoor or outdoor environments. We have ever analyzed various samples collected from hospitals, schools, industrial buildings, etc.
Bacteria, fungi, virus.
Next-generation sequencing (NGS), long-read sequencing, PCR-DGGE, Real-time qPCR, anaerobic culture.
Illumina HiSeq/MiSeq, Ion PGM, PacBio SMRT systems, Nanopore systems, PCR-DGGE, Real-time qPCR, anaerobic culture.
Figure 1. High-throughput sequencing analysis process
Figure 2. PCR-DGGE analysis process
|OTU Clustering||Distribution-Based OTU-Calling|
|Rank Abundance Curve|
|Principal Components Analysis (PCA)|
|Functional analysis||BLASTX, KEGG, eggNOG, CAZy, CARD, ARDB etc.|
|Phylogenetic Analysis||(Un)Weighted Unifrac|