NGS-Based Microbial Identification

We provide 16S metagenomic sequencing and shotgun metagenomic sequencing services for microbial identification. NGS-based microbial identification via single genetic targets or whole-genome strategies allows for the taxonomic assignment of microbial species in complex microbial communities, evolutionary studies, network analysis, and correlation analysis.

• 16S/18S/ITS Sequencing

  16S/18S/ITS metagenomics is an accurate and efficient method for species-level bacterial, archaeal, and fungal identification. Greater resolution (strain-level resolution) may be achieved by sequencing and analyzing the full-length 16S/18S/ITS alleles based on long-read sequencing platforms such as PacBio SMRT sequencing and nanopore sequencing. 16S/18S/ITS metagenomics has been considered as the gold standard for profiling of microbial communities.

• Metagenomics

  Metagenomics is the study of all microbial genomes in mixed communities, allowing the identification of bacteria, eukaryotes, viruses, and pathogens at strain-level resolution. As 16S/18S/ITS sequencing may not be sensitive enough for comprehensive microbiome studies, metagenomics enables the detection of the rare members in the communities. Therefore, metagenomics is a more accurate and sensitive method for microbial identification.

Reference

1. Petrosino J F, Highlander S, Luna R A, et al. Metagenomic pyrosequencing and microbial identification. Clinical chemistry, 2009, 55(5): 856-866.