PASS-based Microbial ARGs Analysis
Antibiotics have been an important part of modern medicine since their introduction. However, the continued overuse and misuse of antibiotics in human and animal health have contributed to the emergence and spread of antibiotic resistance. CD Genomics, the experts in microbial antibiotic resistance gene detection, is committed to providing comprehensive and rapid antibiotic resistance gene (ARGs) analysis services. The Parallel Allele-specific Sequencing (PASS) method detects single base mutations in pathogenic microorganism genomic sequences using solid-phase PCR reactions. It is particularly useful for detecting viral populations with a low percentage of multi-drug resistance genes.
Our Advantages of PASS:
- Wide range of applications: unaffected by viral recombination or resampling.
- High sensitivity: capable of detecting drug-resistant mutated genes in one in 10,000 or even one in 10 million viral populations.
- High throughput: capable of analyzing thousands of viral genomes in a single sample simultaneously.
- Comprehensive information acquisition: can analyze whether drug resistance mutation sites occur in the same genome for chain analysis.
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Introduction to Our PASS-based Microbial Antibiotic Resistance Genes Analysis Platform
The PASS technology works by preparing a mixture of DNA to be tested or cDNA after reverse transcription and polyacrylamide, then performing an in-gel PCR reaction after forming a gel. A clear band forms at the amplification point spot when the product accumulates near the DNA template. Single nucleotides can be labeled with different fluoresceins, primers can be extended by single nucleotides to incorporate bases labeled with different colors, and then mutant types and wild types can be distinguished by scanning imaging. Through multiple single-base extension reactions, PASS technology can detect multiple mutation sites in a single molecule. PASS technology can not only sensitively detect multiple mutation sites on the DNA template, but also judge the existence of different mutation sites in the same genome through parallel analysis, and carry out linkage analysis of mutation sites. It can be used for the detection of most isoforms and recombinants.
PASS-based Microbial Antibiotic Resistance Genes Analysis Workflow
Bioinformatics Analysis
Depending on your needs, we will develop a tailor-made bioinformatics analysis solution for you.
| ANALYSIS CONTENTS | DETAILS |
|---|---|
| Mycobacterium tuberculosis antibiotic resistance loci | Rifampicin resistance-associated loci: 533, 531, 526, 516, 513, 511 |
| HBV drug resistance site | ADV-class agents resistance-associated loci: N236T, A181V |
| L-nucleoside analogs resistance-associated loci: V173L, L180M, M204V, L80V, L80I, A181T, M204I | |
| ETV-based compounds resistance-associated loci: T184G, S202I, M250V, I169T | |
| TDF-class agents resistance-associated loci: A194T | |
| HIV drug resistance loci | NNRTI-class agents resistance-associated loci: L100I, K103N, V106A, V108I, Y181C, Y188L, Y188C, Y188H, G190A |
| d4T-class analogs resistance-associated loci: M41L, D67N, K70R, L210W, T215Y, T215F, K219Q, K219E | |
| Hydroxyinosine resistance-associated loci: K65R, L74V |
Sample Requirements
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- Samples of tissue, serum, or plasma samples are stored at low temperatures (-20℃ or -70℃) immediately after sampling, without repeated freezing and thawing. We do not accept RNA/cDNA samples from you unless you can adequately demonstrate that the RNA/cDNA provided is of high quality.
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