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Antibiotics have been an important part of modern medicine since their introduction. However, the continued overuse and misuse of antibiotics in human and animal health have contributed to the emergence and spread of antibiotic resistance. CD Genomics, the experts in microbial antibiotic resistance gene detection, is committed to providing comprehensive and rapid antibiotic resistance gene (ARGs) analysis services. The Parallel Allele-specific Sequencing (PASS) method detects single base mutations in pathogenic microorganism genomic sequences using solid-phase PCR reactions. It is particularly useful for detecting viral populations with a low percentage of multi-drug resistance genes.
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The PASS technology works by preparing a mixture of DNA to be tested or cDNA after reverse transcription and polyacrylamide, then performing an in-gel PCR reaction after forming a gel. A clear band forms at the amplification point spot when the product accumulates near the DNA template. Single nucleotides can be labeled with different fluoresceins, primers can be extended by single nucleotides to incorporate bases labeled with different colors, and then mutant types and wild types can be distinguished by scanning imaging. Through multiple single-base extension reactions, PASS technology can detect multiple mutation sites in a single molecule. PASS technology can not only sensitively detect multiple mutation sites on the DNA template, but also judge the existence of different mutation sites in the same genome through parallel analysis, and carry out linkage analysis of mutation sites. It can be used for the detection of most isoforms and recombinants.
Depending on your needs, we will develop a tailor-made bioinformatics analysis solution for you.
| ANALYSIS CONTENTS | DETAILS |
|---|---|
| Mycobacterium tuberculosis antibiotic resistance loci | Rifampicin resistance-associated loci: 533, 531, 526, 516, 513, 511 |
| HBV drug resistance site | Adefovir resistance-associated loci: N236T, A181V |
| Lamivudine resistance-associated loci: V173L, L180M, M204V, L80V, L80I, A181T, M204I | |
| Entecavir resistance-associated loci: T184G, S202I, M250V, I169T | |
| Tenofovir resistance-associated loci: A194T | |
| HIV drug resistance loci | Nevirapine resistance-associated loci: L100I, K103N, V106A, V108I, Y181C, Y188L, Y188C, Y188H, G190A |
| Stavudine resistance-associated loci: M41L, D67N, K70R, L210W, T215Y, T215F, K219Q, K219E | |
| Hydroxyinosine resistance-associated loci: K65R, L74V |
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