We offer accurate and affordable whole-genome sequencing of yeast utilizing next-generation sequencing (NGS) or long-read sequencing technologies to detect diverse variations such as structural variations (SVs), insertions and deletions (InDels), single nucleotide variations (SNVs), and copy number variations (CNVs). Both short-read (Illumina platforms) and long-read sequencing platforms (Nanopore and PacBio platforms) are available to achieve whole-genome de novo assemblies and re-sequencing for yeasts.
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Yeast whole genome sequencing represents the determination of the complete DNA sequence of a yeast’s genome in a single time, which entails chromosomal DNA as well as mitochondrial DNA. We utilize both NGS and long-read sequencing platform to provide both whole-genome de novo sequencing and re-sequencing of yeasts. De novo sequencing refers to the sequencing of a novel genome without reference sequence. The coverage quality of de novo assembly predominantly depends on the size and continuity of the contigs. Long-read sequencing including both Oxford nanopore and PacBio sequencing systems enables high-quality genome assembly with improved continuity as the contig N50 length is ten times longer than an NGS-only assembly. Highly accurate NGS data can be used to polish the genome assembly.
While yeast whole-genome de novo sequencing can be used to reconstruct the first genome map for yeast species, providing valuable reference genome for re-sequencing, whole-genome resequencing is commonly used to identify variations such as SNPs, InDels, SVs, CNVs, and other genetic changes, which may be potential biomarkers. Yeast whole genome sequencing provides an unparalleled opportunity to characterize the polymorphic variants in a yeast population, which comprehensively unravels the underlying mechanisms of origin, development, growth, and evolution of yeasts.
Our bioinformatics analysis includes genome assembly, genomic structure analysis, variation detection, functional annotation, comparative genome analysis, and custom analyses.
|Genome assembly||Genome assembly de novo or based on reference alignment with high-quality reads|
|Genomic structure analysis||Genomic island prediction of the genomic island, prophage, CRISPRs, and lncRNAs|
|Variation detection||Detection and annotation SNPs, InDels, CNVs, and SVs|
|Functional annotation||Annotation of NR, Swiss-prot, CAZyme, PHI, KEGG and GO pathway|
|Comparative genome analysis||Synteny analysis, analysis of gene family evolution, evolutionary analysis, ANI analysis|
|Custom analyses||More data mining upon your request|
Sampling kits: We provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.
Deliverables: Raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control report result, statistic data, and your designated bioinformatics report, visualized pictures.