Viral Genome De Novo Sequencing

Viruses negatively impact the health of humans, plants, and animals. The effect of viral pathogens identification can act tremendously on infectious diseases, virology and public health. Based on our viral genome de novo sequencing platform, we help you reconstruct the complete viral genomes and detect genomic mutations, virulence changes, and explore viral evolution. The availability of viral genomes enables researchers to identify unknown pathogens and track variations and diversity of known pathogens.

Our Advantages:

Standardized laboratory with advanced, high-throughput sequencing technology platforms.
Extensive experience in handling various samples.
Keeping up with the frontiers of microbial genomics and bioinformatics analysis.
Next-generation and long-read sequencing platforms for sequencing of complex viral genomes.

Tell Us About Your Project

We are dedicated to providing outstanding customer service and being reachable at all times.

Request a Quote

Introduction to our viral genome de novo sequencing platform

Our viral genome de novo sequencing service leverages the next-generation sequencing (NGS) and long-read sequencing technologies to reconstruct viral genomes. We utilize the advanced illumina HiSeq, Oxford Nanopore, and PacBio SMRT platforms to quickly and efficiently generate high-resolution sequence data. Illumina HiSeq sequencing can offer higher throughput and have lower error rate, while PacBio SMRT and Oxford Nanopore sequencing have the advantage of longer read length, fast assembly, and can detect epigenetic modifications at the same time. Coupled with powerful bioinformatics platforms, we can help you reconstruct draft or complete viral genomes without gaps.

Viral genome de novo sequencing is utilized to discover and characterize viruses, which is crucial to the genetic study of the transmission dynamics, viral disease progression, and viral evolution. Our viral genome de novo sequencing platform can deliver the whole genome sequence and bioinformatics analysis including encoded information, synteny analysis, variation analysis, and evolutionary analysis. Viral genome de novo sequencing is a fast-developing technology with applications in public health monitoring and novel virus discovery.

Viral genome de novo sequencing workflow

Bioinformatics Analysis

Our bioinformatics analysis includes: genome assembly, gene functional annotation, pathogenic microbiology, and comparative genomic analysis. We are flexible to your needs. Feel free to contact us to discuss the bioinformatics pipeline suitable for your project.

Genomic Assembly	De novo genomic assembly and outcome evaluation
	Coding/noncoding gene prediction
	Repetitive sequence analysis
Gene Functional Annotation	NR, GO, COG, KEGG, SwissProt, TrEMBL, Pfam, TCDB, SignalP, CAZy
	Protein coding gene prediction
	Non-coding RNA prediction
Comparative Genomic Analysis	Synteny analysis
	Analysis of gene family evolution
	Phylogenetic analysis
	ANI analysis

Sample Requirement

1. DNA/cDNA, 1.8 < OD_260/280 < 2.0, no degradation or contamination.
2. Illumina platform: concentration > 20 ng, amount ≥ 1 μg.
3. PacBio platform: concentration > 60 ng, amount ≥ 5 μg.
4. Sampling kits
5. We provide a complete range of microbial sampling kits for clients, including microbial collection products, DNA/RNA isolation kits, and accessories for storage and mailing.

Deliverables: Raw sequencing data, assembled and annotated sequences, quality-control dashboard, and the customized bioinformatics report.

References

Kosuri S, Church G M. Large-scale de novo DNA synthesis: technologies and applications. Nature methods, 2014, 11(5): 499.
Marston D A, McElhinney L M, Ellis R J, et al. Next generation sequencing of viral RNA genomes. BMC genomics, 2013, 14(1): 444.

* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.