Fungi are crucial to the biosphere and human health due to their important medical and economic value. Some fungi are closely related to animals, plants, and other life forms by developing symbiotic relationships. Fungi also produce some secondary metabolites like penicillin, cyclosporine, and lovastatin, which have vital medicinal, agricultural, or cosmetic prosperities. Fungal whole genome de novo sequencing can help us better understand the fungal biodiversity, physiology, metabolism, and function. Our fungal whole-genome de novo sequencing based on next-generation sequencing (NGS) and long-read sequencing technologies can assist you to explore novel fungal species in an effective way.
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We utilize both long-read and short-read sequencing technologies for fungal whole genome de novo sequencing. Both PacBio and Nanopore platforms are superior long-read sequencing platforms that generate reads with tens of kilobases in length for better genome reconstruction. A single molecule of DNA can be sequenced without the need for PCR amplification or chemical labeling of the sample. De novo assembly from long reads can span the low complexity and repetitive regions and create accurate assemblies. Additionally, we perform fungal whole genome de novo sequencing on Illumina HiSeq, which is a short-read sequencing platform with high accuracy.
Fungal whole genome de novo sequencing, which is used to explore novel fungal populations, enables the fungal genomes reconstruction, functional analysis, and evolutionary studies. This technique can provide insight into the biological diversity of fungi and yeast, thereby expanding research into agricultural science, ecology, bioremediation, medical science, cosmetic science, bioenergy, and biotechnology industry. We also provide fungal whole genome resequencing to detect variants, explore gene functions, and reconstruct phylogenetic trees.
Our bioinformatics analysis includes three parts: genome annotation, comparative genomic analysis and gene function analysis, which are flexible to your needs.
|Genome Annotation||Repetitive sequence analysis|
|Detection of CRISPRs|
|Non-coding RNA prediction|
|Genomic islands prediction|
|Gene structure analysis|
|Comparative Genomic||Synteny analysis|
|Common and specific genes|
|Gene family anlaysis|
|Gene Function Analysis||Functional annotation|
|Analysis of pathogenicity and drug resistance|
|Prediction of secreted protein|
Sampling kits: We provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.
Deliverables: Raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control dashboard, q-PCR data, statistic data, and your designated bioinformatics report.