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Shallow Shotgun Metagenome Sequencing


Shallow shotgun metagenome sequencing (SSMS) requires sample sequencing at a shallower depth than is used in full metagenome sequencing of shotguns. SSMS is an affordable method to supply compositional and usable sequencing data alike to deep shotgun metagenome sequencing by integrating many samples into a single sequencing run and using a modified protocol that utilizes a lower volume of reagents for sequencing library preparation.

Our Advantages:
  • More efficient functional features and more reliable taxonomic and functional data resolution at roughly the very same expense
  • Appropriate for studies regarding gut microbiome
  • Cheap alternative to 16S sequencing with enhanced taxonomic resolution and functional precision for large-scale biological studies.
  • Appropriate d for routine testing
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Introduction to Our Technology: Shallow Shotgun Sequencing

Shallow shotgun metagenome sequencing (SSMS) requires sample sequencing at a shallower depth than is performed in complete shotgun sequencing. Shallow shotgun sequencing, without the need for traditional deep sequencing, will profile up to 0.05 percent of a microbial population. SSMS provides a smaller representation of the microbial community and higher taxonomic resolution than amplicon sequencing by combining more samples into a single sequencing run and using a modified protocol that uses fewer reagents for sequencing library preparation. SSMS is an economical way to provide a smaller representation of the microbial community and higher taxonomic resolution than amplicon sequencing; SSMS provides composition and function similar (but still not a substitute) to deep-shotgun genome sequencing sequencing data.

Scientists at CD Genomics can access the microbiome with higher species level resolution than 16S sequencing with Shallow Shotgun Sequencing, while still retaining price, making it a perfect alternative for large cohort studies focused on the various microbiomes. We provide you with routine testing, precise functional profiles, reliable taxonomic resolution, and large-scale human microbiome studies. 

Microbial Shallow Shotgun Sequencing Workflow

Bioinformatics analysis

CD Genomics delivers a tiered bundle of study and documentation that can be customized according to the research objectives.

Sequencing QC information

Trimmed, host DNA-depleted, adaptor-removed FASTQ data

Related taxa abundance tables created by mapping to a selected reference database

Functional profiles

Sample Requirement

We accept various sample types, including primary samples, mixed microbial communities and microbial consortia, pre-extracted DNA.

Sampling kits: We provide a complete range of microbial sampling kits for clients, including microbial collection products, DNA/RNA isolation kits, and accessories for storage and mailing.

Deliverables: raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control report result, statistic data, and your designated bioinformatics report, visualized pictures.

* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.

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