Shallow shotgun metagenome sequencing (SSMS) requires sample sequencing at a shallower depth than is used in full metagenome sequencing of shotguns. SSMS is an affordable method to supply compositional and usable sequencing data alike to deep shotgun metagenome sequencing by integrating many samples into a single sequencing run and using a modified protocol that utilizes a lower volume of reagents for sequencing library preparation.
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Shallow shotgun metagenome sequencing (SSMS) requires sample sequencing at a shallower depth than is performed in complete shotgun sequencing. Shallow shotgun sequencing, without the need for traditional deep sequencing, will profile up to 0.05 percent of a microbial population. SSMS provides a smaller representation of the microbial community and higher taxonomic resolution than amplicon sequencing by combining more samples into a single sequencing run and using a modified protocol that uses fewer reagents for sequencing library preparation. SSMS is an economical way to provide a smaller representation of the microbial community and higher taxonomic resolution than amplicon sequencing; SSMS provides composition and function similar (but still not a substitute) to deep-shotgun genome sequencing sequencing data.
Scientists at CD Genomics can access the microbiome with higher species level resolution than 16S sequencing with Shallow Shotgun Sequencing, while still retaining price, making it a perfect alternative for large cohort studies focused on the various microbiomes. We provide you with routine testing, precise functional profiles, reliable taxonomic resolution, and large-scale human microbiome studies.
CD Genomics delivers a tiered bundle of study and documentation that can be customized according to the research objectives.
Sequencing QC information
Trimmed, host DNA-depleted, adaptor-removed FASTQ data
Related taxa abundance tables created by mapping to a selected reference database
We accept various sample types, including primary samples, mixed microbial communities and microbial consortia, pre-extracted DNA.
Sampling kits: We provide a complete range of microbial sampling kits for clients, including microbial collection products, DNA/RNA isolation kits, and accessories for storage and mailing.
Deliverables: raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control report result, statistic data, and your designated bioinformatics report, visualized pictures.