Rhizosphere microorganisms refer to the microorganisms that are closely attached to the rhizosphere soil. They are mainly bacteria, of which gram-negative bacteria including Pseudomonas, Flavobacterium, Pseudomonas, and Agrobacterium are the most predominant microorganisms. Rhizosphere microorganisms are essential for plant growth and organic matter turnover. The plant root exudates are their nutrient and energy source. They mainly affect the permeability and metabolism of root cells. CD Genomics can determine the the types and relative abundance of root microbiomes, explore the microbial diversity of the roots, and study the relationship between the root microbiome and the environment for our clients.Request a Quote
16S/18S/ITS sequencing and metagenomic sequencing are ideal technologies for studying root-associated microbial diversity. According to your specific needs, we employ various sequencing platforms, including Illumina Miseq/Hiseq, Roche 454, Ion Torrent PGM, PacBio RS II, and Nanopore MinION instruments to obtain huge and accurate sequence data. Comprehensive bioinformatics approaches such as RDA, CCA, NMDS analysis are used to investigate the microbial community structure, the relationship between environmental factors (pH, C/N, humidity, total nitrogen, total phosphorus, TOC, water content, etc.) and the root microbiome, and the relationship between root microorganisms and plant growth.
Note: Our service is for research use only, and not for therapeutic or diagnostic use.
Bacteria, fungi, archaebacteria.
Next-generation sequencing (NGS), long-read sequencing, PCR denaturing gradient gel analysis (PCR-DGGE), real-time qPCR
Illumina HiSeq/MiSeq, Ion Torrent systems, PacBio SMRT systems, Nanopore MinION systems, PCR-DGGE, Real-time qPCR, clone library, etc.
Figure 1. Our workflow for high-throughput sequencing analysis.
Figure 2. Workflow for PCR-DGGE analysis process.
|OTU Clustering||Distribution-Based OTU-Calling|
|Rank Abundance Curve|
|Principal Components Analysis (PCA)|
|Functional analysis||BLASTX, KEGG, eggNOG, CAZy, CARD, ARDB etc.|
|Phylogenetic Analysis||(Un)Weighted Unifrac|