MicroCollect™ Saliva RNA Collection Device

An all-in-one system for the collection of high-quality salivary RNA

Saliva sampling is simple and noninvasive, and can be used for extracellular RNA (exRNA) biomarker discovery and molecular diagnosis by utilizing RNA analysis approaches such as RNA-seq. Salivary RNA analysis creates a new paradigm in the field for noninvasive molecular diagnosis. Compared with salivary DNA, salivary RNA is more labile, especially due to the ribonucleases present in saliva. To solve this problem, we have identified some macromolecules that can protect salivary RNA from ribonucleases.

MicroCollect™ Saliva RNA Collection Devices is a cylindrical device with spiral interface funnel. It is convenient to collect saliva by directly spitting into non-toxic buffer preservative solution until the amount of saliva reaches the fill line. The funnel is then removed and the lid is screwed on. Saliva samples can be used in biochemical and molecular analysis. This device can collect and preserve superior oral samples for molecular detection of RNA, such as RT-PCR, RT-qPCR, RNA sequencing and microarray.

Features

• User-friendly, non-invasive saliva collection.
• Sampling can be done at home or other places without any special medical training.
• Widely used at home and R&D laboratories for scientific discoveries, translational research.
• Suitable for a range of downstream applications, such as RT-PCR, RT-qPCR, RNA sequencing and other molecular experiments.
• Sample collection and preservation in one convenient kit.
• The sample can be preserved at room/frozen temperature and high quality RNA can be isolated.
• The preservative buffer inhibits bacterial growth.
• Unique funnel design prevents spillages or buffer flow back.

Instructions for Use

Each MicroCollect™ Saliva RNA Collection Device consists of 3 components: (1) collection funnel and collection tube, (2) preservative, and (3) tube cap. The instructions for use are as follows:

1. Spit the saliva into the collection funnel until the amount of saliva reaches the fill line in the condition of the clean mouth.
2. Close the collection funnel lid tightly without any saliva and unscrew the funnel from the tube.
3. Use the tube cap to close the collection tube tightly.
4. Oscillate the collection tube for 10 seconds.
5. The sample is ready for downstream analysis.

Specification