Many microorganisms, including bacteria, viruses and fungi, coexist in the lungs of humans or animals and constitute the respiratory microbiota. It is generally accepted in the scientific community that the lung or respiratory microbiota is less biologically abundant, scarcer, and associated with mucosal surfaces than the relatively stable intestinal community. In this dystrophic microbial environment, high-throughput sequencing technologies can not only capture signals with exceptionally low DNA/RNA densities and characterize a variety of novel in samples but can also simultaneously study pathogen and host responses through molecular approaches such as microbial metagenomics and transcriptomics.
CD Genomics is dedicated to breaking down the limitations of lung microbiome research by NGS and long-read sequencing platforms, processing or speed to help you generate and comprehend high-dimensional data.Request a Quote
We can provide solutions for respiratory and lung microbiology research and bioinformatics analysis in combination with powerful sequencing technologies, based on next-generation sequencing (NGS), PacBio SMRT sequencing and nanopore sequencing platforms. In addition, we perform qualitative and quantitative studies of the microbiome using real-time qPCR.
1. Characterization of the respiratory microbiome
2. Quantitation of the respiratory microbiome
3. Revealing a wealth of information about community composition and dynamics
Note: Our services are for research use only, not for disease diagnosis and treatment.
Sputum, alveolar lavage fluid, bronchial brushings and lung tissue, and other lung microbiome sample types
Bacteria, fungi, viruses, etc.
We are equipped with Illumina Hiseq/Miseq, PacBio SMRT systems, Nanopore systems, PCR-DGGE (PCR-denaturing gradient gel electrophoresis), real-time qPCR, and other detection platforms.
Be sure that the DNA is intact and non-degradative, avoiding repeated freezing and thawing cycles.
Please use enough dry ice or ice packs during shipment.
Please contact us for more details about the sample preparation for respiratory and lung microbiome sequencing.
Figure 1. High-throughput sequencing analysis process
|BASIC ANALYSIS||ROUTINE ANALYSIS (ACCORDING TO CUSTOMER REQUIREMENTS)||ADVANCED DATA ANALYSIS|
|Sequence Filtering and Trimming||Heatmap||Phylogenetic Tree|
|Sequence Length Distribution||VENN||LDA-Effect Size (LEfSe)|
|OTU Clustering and Species Annotation||Principal Components Analysis (PCA)||Network Analysis|
|Diversity Index||Microbial Community Structure Analysis||Correlation Analysis|
|Shannon-Wiener Curve||α Diversity Index Analysis|
|Rank-Abundance Curve||Matastats Analysis|
|Rarefraction Curve||Weighted Unifrac test|
|Multiple Contrast||CCA/RDA Analysis|
|Principal Components Analysis (PCA)|