We offer comprehensive metagenomics service based on a single molecule, real-time (SMRT) sequencing from PacBio Biosciences. The SMRT-based metagenomics is a culture-free, long-read sequencing strategy that can effectively reduce some splicing errors and obviously improve the resolution of microbial community profiling. This service aims to examine thousands of microorganisms in parallel and comprehensively obtain all genes, providing insight into community biodiversity and function.
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Microorganisms are almost everywhere. However, more than 99% of them cannot be cultured in vitro. Our SMRT-based metagenomics is a highly accurate, culture-independent metagenome profiling approach for studies of the microbial communities using PacBio RSII or Sequel from PacBio Biosciences. The PacBio SMRT sequencing technology is characterized by unparalleled read length and continuously increasing accuracy, which is helpful for the reconstruction of the high-quality draft and complete metagenomic analysis.
SMRT-based metagenomics can be used to study the species composition and abundance, identify differentially expressed genes (DEGs) among samples, explore gene functions and metabolic pathways, and mine gene resources for bioactive products. The technology can also be used to elucidate and understand the relations between microbes and their habitat/host, like the microenvironments within the human body in healthy or diseased states. SMRT-based metagenomics has been widely applied to a range of fields, including research, clinical use, biotech industry, etc.
Our bioinformatics analysis includes four parts: raw data processing, assembly, binning, annotation. We are flexible to your needs.
|Raw data processing||Filtering and trimming of poor-quality sequence.|
|Assembly||Reference-based assembly, de novo assembly, and quality assessment of metagenomics assemblies.|
|Binning||Phylogenetic binning using tools like LikelyBin, PHYSCIMM, MetaWatt, CONCOCT, etc.|
|Annotation||Metagenome gene prediction using tools like MetaGeneAnnotator, Orphelia, and Glimmer-MG; protein function prediction using InterProScan software pipeline; pathway annotation using databases like KEGG, WikiPathways, and MetaCyc.|
Sampling kits: We provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.
Deliverables: Raw data files in BAM format, demultiplex CCS reads in FASTQ format, quality-control dashboard, statistic data, and your designated bioinformatics analysis report.