We provide high-throughput sequencing of archaeal 16S rRNA genes. Coupled with bioinformatics analysis, we help researchers investigate archaeal diversity, abundance, and phylogeny on a global scale. This service is dedicated to assisting our customers in meeting both scientific and regulatory requirements.
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The 16S rRNA gene is a highly conserved component of all DNA-based life forms and thus is highly suited as a molecular marker for sequencing DNA in samples containing thousands of different species. Because of the difficulty of cultivating archaeal compared with bacteria, archaeal 16S rRNA sequencing is a preferred method for the understanding of the origin and evolutionary relationship of archaeal. We own a range of advanced next-generation sequencing (NGS) platforms, such as Illumina Hiseq2500, HiSeq 4000, Miseq, and Roche 454 GS FLX platform, as well as third-generation sequencing platforms such as PacBio RS II and Nanopore instruments. Based on our diverse sequencing platforms and expertise in microbial genomics, we are dedicated to providing (full-length) 16S rRNA gene sequencing for identification, classification, and quantitation of archaeal within complex biological mixtures such as environmental samples.
We provide comprehensive bioinformatics analyses including original sequence quality control, OUT clustering, taxonomic classification, diversity analysis, abundance analysis, and phylogenetic analysis. Functional analysis can also be carried out to comprehensively analyze the biological significance of archaea, such as their beneficial or potentially pathogenic effects in the human microbiome. We can guide you to discover new archaea and understand their biological functions. At present, there are only less than 1000 archaea genomic data in the National Center of Biotechnology Information (NCBI) database, which is slightly insufficient compared with the actual biodiversity. Since archaea are found in soils, the human bodies, and extreme environment, archaea has important implications for human health, animal and plant health, and industrial applications, etc.
Our bioinformatics analysis includes five parts: species annotation, diversity analysis, evolutionary analysis, functional profiling, and correlation analysis. We are flexible to your needs.
|Bioinformatics Analysis||The Problem to be Solved|
|Quality control||Read quality filtering, quality trimming, OTU filtering|
|Species annotation||OTU clustering, species annotation|
|Diversity analysis||α diversity||Diversity index; rarefaction curve|
|β diversity||Sample differences|
|Meta-analysis||Comparative studies of abundant features between datasets|
|Evolutionary analysis||Construction of phylogenetic trees|
|Functional analysis||Prediction of the biological significance of archaea|
|Custom analysis||Custom analysis based on customer needs|
Sampling kits: We provide a complete range of microbial sampling kits for clients, including microbial collection products, DNA/RNA isolation kits, and accessories for storage and mailing.
Deliverables: Raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control dashboard, q-PCR data, statistic data, and your designated bioinformatics report.