Biofilms refer to the bacterial communities that attach to surfaces. They contribute to the wellbeing and resilience of ecosystems via their important roles in biogeochemical cycling, their interactions with each other and their provision of chemicals. Although aquatic biofilms are important to the health of marine ecosystems, they also cause deleterious outcomes like biocorrosion, biofouling and antibiotic resistance. When it comes to human health, microbes can form biofilms on any surface in the human body and cause resistance to antimicrobial agents, triggering persistent and chronic bacterial infections. Biofilm infections have sharply come into prominence in the last few decades. Next-generation sequencing (NGS) is a powerful tool when studying the biofilm microbiomes. Genomic studies have revealed the complexity and diversity of the biofilms.Request a Quote
We provide advanced genomics methods to explore the microbial diversity and structure of biofilm samples, and discover previously undetected microbial species and predict their roles in biofilm formation, human health or environments. Our solutions include real-time PCR, 16S/18S/ITS sequencing, metagenomics, metatranscriptomics and powerful bioinformatics analysis. Based on Illumina Hiseq/Miseq, PacBio and Oxford nanopore sequencing platforms, we are dedicated to ensuring an accurate & comprehensive microbial profiling and elaborate bioinformatics analysis pipeline with standardized laboratory methods.
Note: our service is for research use only, not for diagnosis.
Artificial biofilms, marine microbial biofilms and other biofilms on any surface.
Archaea, bacteria, eukaryotes, etc.
We are equipped with Illumina Hiseq/Miseq, PacBio Sequel, Oxford MinIONTM, Oxford GridIONTM, PCR-DGGE (PCR-denaturing gradient gel electrophoresis), real-time qPCR, clone library and other platforms.
Figure 1. High throughput sequencing analysis process
Figure 2. PCR-DGGE analysis process
Our bioinformatics analysis pipeline is flexible to your needs.
|Basic Analysis||Routine Analysis (According to Customer Requirements)||Advanced Data Analysis|
|Read QC||Heatmap||Phylogenetic Tree|
|Sequence Length Distribution||VENN||Functional Core Analysis|
|OTU Clustering and Taxonomic Profiling||Principal Components Analysis (PCA)||LDA-Effect Size (LEfSe)|
|Diversity Index||Microbial Community Structure Analysis||Network Analysis|
|Shannon-Wiener Curve||α Diversity Index Analysis||Correlation Analysis|
|Rank-Abundance Curve||Matastats Analysis|
|Rarefraction Curve||Weighted Unifrac test|
|Principal Components Analysis (PCA)|