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Viral Metagenomics

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Overview

The aim of our viral metagenomics is to study viruses efficiently and easily by utilizing the next-generation sequencing technology and random-primed PCR, coupled with comprehensive bioinformatics analysis.

Our Advantages:
  • Nearly all known and novel viruses in the sample can be analyzed using high-throughput sequencing and random PCR.
  • Culture-independent. The viruses are obtained directly from the environment.
  • We can systematically analyze and identify viruses which are too scattered or rare in the sample.
  • We provide comprehensive information on the biodiversity, taxonomy, and function of viruses in the sample.
  • Extensive experience and professional analysis.
Tell Us About Your Project

We are dedicated to providing outstanding customer service and being reachable at all times.

Request a Quote

Introduction to our viral metagenomics

Viral metagenomics is the application of environmental genomics in viruses, which needs a combination of innovative technologies such as viral operations, random PCR, next-generation sequencing (NGS), and long-read sequencing. The common sequencing platforms for viral metagenomics contain Illumina HiSeq/MiSeq, PacBio RS II, and Nanopore SMRT instruments. The technical routes include sample preparation, enrichment of viral particles, DNA libraries construction, sequencing, and bioinformatics analysis. The efficiency of viral metagenomics and bioinformatics analysis has been greatly improved in recent years. Viral metagenomics surpasses traditional viral research for its ability to detect low-abundance viruses.

Viral metagenomic studies have provided insights into viral ecology by elucidating the genetic potential, community structure, and biogeography of environmental viruses. Viral metagenomics has expanded current knowledge on virus-host interactions by uncovering genes that may allow the viruses to manipulate their hosts in unexpected ways. The intrinsic potential for virus discovery through viral metagenomics can help advance a wide array of disciplines including evolutionary biology, pathogen surveillance, and biotechnology. In addition, functional viral metagenomics serves as a platform to discover more useful enzymes. As the annotation of viral genes continues to improve, the discovery of vital enzymes will increase considerably.

Viral metagenomics workflow

Bioinformatics Analysis

Our bioinformatics analysis includes five parts: quality control, genomic assembly, taxonomic assignment, diversity analysis, functional analysis, and custom analysis. For advanced bioinformatics analysis in detail, please refer to the following table.

Bioinformatics Analysis Details
Quality control Fastqc, cutadapt, fastp, etc.
Genome assembly Genovo, Bambus 2, Spades, MetaVelvet, Meta-IDBA, MEGAHIT, Ray, et al.
Taxonomic assignment NCBI nr/nt database; Bowtie, BWA, SOAP3, mrsFAST, et al.
Diversity analysis PcoA, NMDS, Heatmap, Krona analysis, clustering tree, etc.
Functional analysis ARDB, CAZY, KEGG, eggNOG, GO, MG-RAST, IMG/M, WebMGA, RAMMCAP, etc.
Custom analysis RDA/CCA, correlation analysis, ROC curve, etc.

Sample Requirement

    1. DNA amount ≥ 3 μg, concentration ≥ 30 ng/μL, 1.8 < OD260/280 < 2.0.
    2. Non-degraded DNA is placed into DNase-free tubes with clear labels. Please use dry ice during transport.

Sampling kits: We provide a complete range of microbial sampling kits for clients, including microbial collection products, DNA/RNA isolation kits, and accessories for storage and mailing.

Deliverables: Raw sequencing data (FASTQ), quality-control dashboard, statistic data, and your designated bioinformatics report.

* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.



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