The extreme environment such as volcanoes, glaciers, and deep seas, is an indispensable part of the natural environment. Its condition is too harsh for human, plants, and animals to exist. However, there is probably a large number of microorganisms living in such places, which are called extremophiles. These microorganisms represent the limits to adapt to the environment and are treasures of biological genetics and functional diversity. They not only play an important role in the origin and evolution of life, but also have great applications in studying specialized genotypes, physiological mechanisms and metabolites, revealing the mechanism of these microorganisms to colonize hostile ecosystems. The enzymes isolated from them are currently being exploited for their commercial interest and industrial applications. At present, extreme microbes have become a hot field of international research.Request a Quote
With the development of next-generation sequencing and bioinformatics tools, it is possible and much easier to conduct systematic classification, gene function analysis, and pathway analysis in extreme environments. We provide a broad array of microbial genomics solutions to help researchers explore the diversity of microbial community structures in extreme environments and maybe find resources that have great potential for industrial applications.
16S ribosomal RNA (16S rRNA), the most common housekeeping genetic marker in bacteria, is ideal for bacterial detection and bacterial diversity analysis. 18S rRNA and ITS are ideal for fungal detection and diversity analysis. We provide both short-read and full-length 16S/18S/ITS amplicon sequencing and qPCR services for microbial identification and quantification in extreme environments.
We utilize 16S/18S/ITS amplicon sequencing and metagenomics to help you simultaneously detect dominant species, rare species, and unknown species in your sample, and obtain the microbial community composition and relative abundance. In order to improve identification accuracy and alignment rate, we also provide long-read sequencing services utilizing PacBio SMRT sequencing or Nanopore sequencing.
After sequencing, we process the data through our powerful bioinformatics analysis platform. First, the raw data is filtered out or trimmed, and the qualified data is retained for alignment, assembly, taxonomic assignment, diversity analysis, phylogenetic analysis, network analysis, correlation analysis, functional prediction and so on.
Note: Our service is for research use only, and not for therapeutic or diagnostic use.
Bacteria, eukaryotes, archaebacteria.
Next-generation sequencing, third-generation sequencing, PCR denaturing gradient gel analysis (PCR-DGGE), real-time PCR
Illumina HiSeq/MiSeq, Ion PGM, PacBio sequencing, Nanopore sequencing, PCR-DGGE, Real-time qPCR, clone library etc.
DNA ≥ 300 ng, 1.8<OD260/280<2.2, concentration ≥ 10 ng/μl, Volume ≥ 10 μl.
Ensure that the DNA is not degraded or slightly degraded. Avoid repeated freezing and thawing during sample storage and shipment.
Please use enough dry ice or ice packs during shipment.
Nucleic acid samples should be stored at -20 °C.
Figure 1. High-throughput sequencing analysis process
Figure 2. PCR-DGGE analysis process
|Basic Analysis||Routine Analysis (According to Customer Requirements)||Advanced Data Analysis|
|Sequence Filtering and Trimming||Heatmap||Phylogenetic Tree|
|Sequence Length Distribution||VENN||LDA-Effect Size (LEfSe)|
|OTU Clustering and Species Annotation||Principal Components Analysis (PCA)||Network Analysis|
|Diversity Index||Microbial Community Structure Analysis||Correlation Analysis|
|Shannon-Wiener Curve||α Diversity Index Analysis|
|Rank-Abundance Curve||Matastats Analysis|
|Rarefraction Curve||Weighted Unifrac test|
|Multiple Contrast||CCA/RDA Analysis|
|Principal Components Analysis (PCA)|