We provide fungal ITS sequencing service using either next-generation sequencing or long-read sequencing platforms, to help you identify novel fungal species and explore the structure of fungal communities and the roles of fungi in the environment or in/on the human body.
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Internally Transcribed Spacer (ITS) lies between highly conserved nuclear ribosomal DNA (rDNA) genes (18S, 5.8S, 28S). It tends to be hypervariable and unique among species but fairly unchanged among individuals from the same species, so it is a suitable marker for species identification. ITS can reflect differences between fungal genera, species, and even strains. In addition, the length of ITS sequence fragments (the lengths of ITS1 and ITS2 are 350 bp and 400 bp respectively) is short, which is easy to amplify, sequence, and analyze, and containing sufficient interspecific polymorphisms. Our fungal ITS sequencing using advanced NGS platforms (Illumina PE250/300) is ideal for identification and quantitation of known fungi, and discovery of novel fungi.
Fungal ITS sequencing can be used to identify the diversity of fungi in a range of samples, and investigate their biological significance. With the emergence of fungi that are resistant to the antifungal drugs available, fungal ITS sequencing has therefore become essential for the rapid and accurate identification of fungi in a routine diagnostic microbiology laboratory. Fungal ITS sequencing can also be used for fungal plant disease diagnoses. Fungal ITS sequencing has been widely applied to a range of fields, including environmental conservation, agricultural production, petroleum exploration, and industrial manufacturing, etc.
After DNA sequences are obtained, bioinformatics tools such as QIIME and mothur are commonly used for raw data processing, taxonomic assignment, diversity and richness analysis, comparative analysis, and evolutionary analysis. We are flexible to your needs.
|Raw data processing||Filtering and trimming of poor-quality sequences, read alignment|
|Taxonomic assignment||Operation Taxonomic unit (OTU) clustering and taxonomic assignment|
|Diversity and richness analysis||Rank abundance, Venn, alpha diversity analysis, beta diversity analysis, rarefaction curves, heatmap, etc.|
|Comparative analysis||Metastats, LEfSe, PCA analysis, PCoA analysis, etc.|
|Evolutionary analysis||(Un)weighted UniFrac analysis, phylogenetic trees|
|Custom analysis||CCA analysis, PICRUSt, network analysis, etc.|
Sampling kits: We provide a complete range of microbial sampling kits for clients, including microbial collection products, DNA/RNA isolation kits, and accessories for storage and mailing.
Deliverables: Raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control dashboard, statistic data, and your designated bioinformatics report.