Fungal whole genome resequencing refers to the high-throughput sequencing of fungal individuals or populations with the availability of a reference genome. We provide the fungal whole genome resequencing platform to help you mine a large number of single nucleotide polymorphisms (SNPs), insertions and deletions (InDels), structural variations (SVs), and copy number variations (CNVs) in the fungal genomes.
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We provide fungal whole genome resequencing using a whole-genome shotgun strategy by next generation sequencing (Illumina HiSeq PE150/251, 100X) or long-read sequencing platforms (PacBio RSII or Nanopore MinION instrument). A large amount of reads generated from sequencers are subjected to quality control, and the clean data are subsequently aligned to reference genome sequences. With powerful bioinformatics analysis, we can detect a large number of variations including SNPs, InDels, SVs, CNVs, etc. Fungal whole genome resequencing is an effective, accurate, and rapid method for sequencing and analyzing fungal genomes.
With the increasing of high-quality reference genome sequences that are available for different species, the application of fungal genome resequencing become more widespread. Fungal whole genome resequencing can quickly and efficiently detect both mutations and structural variations on a global genome. Comparative genomic analysis is provided to identify differentially expressed genes through gene ontology analysis and explore the evolutionary relationships and biological traits, including pathogenic mechanism and resistant mechanism. Moreover, our platform supports multiple fields, including pharmaceutical and biotechnology industries, scientific research, clinical applications, agriculture, etc.
Our bioinformatics analysis includes: genomic assembly, genomic structure studies, functional annotation and comparative genomic analysis. For advanced bioinformatics analysis in detail, please refer to the following table.
|Genomic Assembly||Data quality control|
|Raw data filtering|
|Genomic Structure Analysis||Detection of SNPs, InDels, SVs, CNVs|
|Detection of CRISPRs|
|Functional Annotation||NR, GO, COG, KEGG, SwissProt, Pfam, TCDB, SignalP, CAZy|
|Comparative Genomic Analysis||Synteny analysis|
|Analysis of gene family evolution|
Sampling kits: We provide a complete range of microbial sampling kits for clients, including microbial collection products, DNA/RNA isolation kits, and accessories for storage and mailing.
Deliverables: Raw sequencing data (FASTQ), annotated sequences (FASTA), quality-control dashboard, statistic data, and the bioinformatics report.