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Microbial DNA Metabarcoding

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CD Genomics uses next-generation sequencing technologies to provide microbial DNA metabarcoding sequencing and bioinformatics services. With cutting-edge technologies, we can fully assist with your microbial ecology studies.

Our Advantages
  • Extensive experience in sequencing
  • Compatible even with degraded DNA samples
  • Comprehensive bioinformatics supports
  • Supporting basic and applied research
DNA 6mA Sequencing Service
Tell Us About Your Project

We are dedicated to providing outstanding customer service and being reachable at all times.

DNA 6mA Sequencing Service Request a Quote

Introduction to Our Microbial DNA Metabarcoding Services

DNA metabarcoding is a sequencing technology based on amplicon profiling. While conventional DNA barcoding focuses on a specific species, metabarcoding can detect the whole communities. The species information is obtained from a reference database containing DNA barcodes, which can be applied to microbial ecology research. Microbial environmental DNA metabarcoding (eDNA metabarcoding) refers to a method for high-throughput DNA sequencing used for simultaneous molecular identification of multiple taxa in a complex environmental sample (such as soil, water, sediments, and feces). In recent years, this method has attracted wide attention from scientists, and has gradually been applied to biodiversity research, biological monitoring, and many other fields.

CD Genomics provides DNA metabarcoding service to investigate the microbial diversity of environmental samples. Based on our illunima next-generation sequencing platform, we provide comprehensive and reliable microbial DNA barcoding and sequencing services, and help you identify microorganisms down to various taxonomic levels and compare the diversity among multiple samples.

Our sequencing platforms can generate millions of sequences, enabling the study of complex environmental samples in a short period of time. Metabarcoding uses primers to amplify DNA in the environment, and high-throughput sequencing obtains the large-scale taxonomic information contained in the genes. We provide fine-grained data analysis of a large number of sequence fragments from complex environmental samples, mainly including: noise removal, obtain operational taxonomic unit (OTU) data, and subsequent analysis based on OTUs.

Microbial DNA Metabarcoding Workflow

Microbial DNA Metabarcoding Workflow

Bioinformatics Analysis

Our bioinformatics services offer scientists custom analysis to address real issues apart from data quality control, gene alignment, genome assembly, and phylogenetic analyses. Our bioinformatics analysis services provide comprehensive interpretations of environmental DNA (eDNA) metabarcoding. For more details, please refer to the following table.

Table 1. Our custom bioinformatics analysis for microbial DNA metabarcoding analysis.

Taxonomic assignment Reference-based methods are used for the precise taxonomic assignment of sequencing reads.
Metabolic analysis Metabolic processes encoded in the genome, from biosynthesis to biodegradation, directly link microbial communities to the environment.
Habitat prediction Predict possible habitats of microbes.
Genome reconstruction Reconstruction of microbial genome by metagenomic binning and assembly from various environments.
Inter-species interactions Use abundant information from large-scale metagenomic datasets, co-occurrences (or anti-occurrences) among microbes, hosts, and/or viruses can be studied.
Genomic structural variations Observe systematic variations in the gene order (or gene cluster structures) due to gene losses, fusions, duplications, inversions, translocations, and HGTs from an analysis of metagenome data.
Meta-analysis Meta-analysis of multiple datasets can reveal general patterns or laws that determine how microbes interact with their environments and how their genomes have been shaped.

Sample Requirements

  • Microbial DNA Metabarcoding

    1. No obvious degradation, 1.8 < OD260/280 < 2.0, OD260/230 ≥ 1.8, DNA/cDNA amount ≥ 1 ug
    2. Deliverables: raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control dashboard, statistic data, and your designated bioinformatics report.
* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.

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