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Antibiotic Resistance Genes (ARGs) Analysis Solution
DNA Microarray-based ARGs Analysis
Mycobacterium tuberculosis ARGs Analysis

Mycobacterium tuberculosis ARGs Analysis

Today, antibiotic resistance genes have become an important issue of global public health concern and the biggest challenge facing infectious diseases today. Tuberculosis (TB) is a serious public health problem worldwide, and multidrug-resistant Mycobacterium tuberculosis and extensively drug-resistant Mycobacterium tuberculosis are one of the problems that need to be solved urgently. Therefore, rapid and accurate detection of drug-resistant Mycobacterium tuberculosis (MTB) in specimens is crucial to effectively control the tuberculosis epidemic. CD Genomics provides DNA microarray-based detection of antibiotic resistance genes (ARGs) in Mycobacterium tuberculosis.

Our Advantages of Mycobacterium tuberculosis ARGs Analysis:

Eliminates the need for bacterial culture and greatly reduces the time required for antibiotic resistance gene testing.
Provides a basis for laboratory studies of Mycobacterium tuberculosis and reduces the spread of drug-resistant strains.
Helps to study the epidemiological distribution of MTB resistance and provides a reference basis for the prevention and treatment of Mycobacterium tuberculosis.
The use of gene chip technology to detect antibiotic resistance gene mutations avoids false negatives, and the results are accurate and reliable.

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What We Offer

Mycobacterium tuberculosis ARGs Analysis

Our DNA microarray-based platform can rapidly and simultaneously detect multiple resistance-related genes and can detect multiple mutation sites of these resistance genes. The study found that Mycobacterium tuberculosis was mainly resistant to antibiotics such as rifampicin, isoniazid, and streptomycin. The services we can provide to customers include but are not limited to the following.

Rifamycin resistance gene detection: Rifampicin (RFP), rifapentine (RFT) and rifabutin (RFB) are all first-line anti-TB drugs. Our detection service can target the mutation of rpoA, rpoB, and rpoC.
Isoniazid and isonicotinic acid derivative resistance gene detection: Our detection service can target the deletion or mutation of katG, inhA, and ethA.
Ethambutol resistance gene detection: Our service targets overexpression or mutation of the arabinosyltransferase encoding genes embA, embB, and embC.
Pyrazinamide resistant genes detection: Our service can target mutations in the pyrazinamide-encoding gene pncA, ribosomal protein S1-encoding gene rpsA, and aspartate decarboxylase gene panD.
Streptomycin resistance gene detection: Our service can target the mutations of ribosomal protein S12 coding gene rpsL and 16S rRNA coding gene rrs. Focus on mutations in RpsL43 and 88 codons and rrs905 and 513 nucleotides.
Quinolones resistance gene detection: Our service can target mutations in the A subunit encoding gene gyrA or the B subunit encoding gene gyrB.
Kanamycin, capreomycin, and viomycin resistance gene detection: Our service can target mutations in the rrs and mutations in tlyA.
Macrolide resistance genetic detection: Our service can target mutations in ermMT and Tap.
Cycloserine resistance gene detection: Our service can target mutations in the genes gadA, alrA, ddlA, and ald.

Data Analysis

Data Analysis：Data analysis for detection of antibiotic resistance genes in Mycobacterium tuberculosis - CD Genomics.

Sample Requirements

SAMPLE TYPE	SAMPLE DETAILS
Sputum	1 mL or more. For transport, use biological ice packs (2-8℃)/3 d. The container is chosen to be in a sterile airtight sputum box.
Alveolar lavage fluid	2-3 mL. For transport, use biological ice packs (2-8℃)/3 d. The container is chosen to be in a sterile airtight sputum box.
Purulent secretions	2-3 mL. For transport, use biological ice packs (2-8℃)/3 d. The container is chosen to be in a sterile airtight sputum box.
Puncture fluid	2-3 mL. For transport, use biological ice packs (2-8℃)/3 d. The container is chosen to be in a sterile airtight sputum box.
Urine	2-3 mL. For transport, use biological ice packs (2-8℃)/3 d. The container is chosen to be in a sterile airtight sputum box.

Reference

Tang X, Morris SL, Langone JJ, et al. Microarray and allele specific PCR detection of point mutations in Mycobacterium tuberculosis genes associated with drug resistance. J Microbiol Methods. 2005 Dec; 63(3): 318-30.