We offer single molecule, real-time sequencing (SMRT)-based microbial targeted and whole-genome sequencing for precise microbial identification, the generation of complete reference genomes, comparative genomic studies, and genomic exploitation. And the approach has a diverse set of applications, such as accurate microbial identification, microbial genomic characterization, pathogen detection, environmental monitoring, and routine testing of raw materials for bacterial contamination.
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The Microbial genome is smaller than eukaryotes. Most bacteria have only one circular chromosome, while some bacteria have more than one chromosome (several linear chromosomes or combinations of circular and linear chromosomes). The presence of plasmids (the circular extra-chromosomal DNA and the bacterial genome) is another major character of prokaryotic genomes, which mediate the rapid evolution of microorganisms and can be transferred by horizontal DNA transfer. Our SMRT-based genome sequencing can provide more accurate and contiguous sequences for complete bacterial, viral, and fungal DNA or RNA genomes with SMRT technology from PacBio Biosciences. The PacBio SMRT sequencing technology is characterized by unparalleled raw read accuracy, read length and read depth, which is helpful for the reconstruction of high-quality drafts and complete microbial genomes.
Based on the PacBio SMRT platform, we provide microbial targeted and whole-genome sequencing services to analyze challenging genomic regions like structural variants and repeat sequences for high-quality assemblies, perform accurate microbial identification, clarify the role of phage insertion, transposon insertion and other structural variants in the evolution of virulence, and recover plasmids to track transmission paths and drug resistance. Our SMRT-based genome analysis can be used to redefine the genetic and biochemical diversity in complex communities, elucidate virus-host interactions, uncover new taxonomic groups and metabolic capabilities, and determine how functions are distributed across environmental gradients.
Our bioinformatics analysis includes raw data quality control, genome annotation, gene function annotation, reference genome alignment, and custom analysis, which is flexible to your needs.
|Raw data quality control||Correction, classification, reduced redundancy, etc.|
|Genome annotation||Prediction of pathogenicity genes, susceptibility gene prediction, non-coding RNA prediction, detection of clustered regularly interspaced short palindromic repeats (CRISPRs), etc.|
|Gene function annotation||COG/GO/KEGG analysis, etc.|
|Comparative genomics||Construction of phylogenetic trees, divergence-time estimation, etc.|
|Custom analysis||More data mining upon your request.|
Sampling kits: We provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.
Deliverables: Raw data files in BAM format, demultiplex CCS reads in FASTQ format, quality-control dashboard, statistic data, and your designated bioinformatics analysis report.