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Phage Whole-Genome Sequencing 

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Overview

CD Genomics uses next-generation sequencing and long-read sequencing technologies (mainly Illumina HiSeq, Nanopore, and PacBio SMRT sequencing) to provide virus/phage sequencing services, and help your in-depth study of structural genomics and comparative genomics. Based on the virus genome sequencing platform, we provide resequencing and de novo sequencing strategies to construct draft or complete virus/phage genomes.

Our Advantages
  • Extensive experience in sequencing
  • Multiple platforms include NGS and TGS
  • Comprehensive bioinformatics supports
  • Supporting basic and applied research
DNA 6mA Sequencing Service
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Introduction to Our Phage Whole Genome Sequencing Platform

Phages are a group of viruses that invades bacteria, some of which can cause the lysis of the host bacteria, and some can also give the host bacteria the genetic material for extra biological characteristics. Bacteriophages “attack” bacteria and have strict host specificity, which depends on the molecular structures and complementarity of the phages and the receptors on the surface of the recipient bacteria. Bacteriophages are the most common and widely distributed viruses, which are usually abundant in places full of bacterial communities, such as soil and the gastrointestinal tract. Bacteriophages are the research object that cannot be ignored when studying microecology. And these researches provide industry opportunities that deserve attention.

Based on our second-generation sequencing and third-generation whole-genome sequencing platforms, we provide comprehensive and reliable phage whole-genome de novo sequencing, resequencing, and genetic analysis services. These sequencing technologies are also the tools to understand the biochemical interaction between prokaryotes and phages, and have been widely used in the food industry and medical research. For example, multiple drug resistance (MDR) has risen dramatically, and phage therapy may be an effective way to treat bacterial infections. Our phage sequencing service can help you discover biomarkers and develop non-antibiotic treatment methods.

Phage Whole-Genome Sequencing Workflow

Phage Whole-Genome Sequencing Workflow

Bioinformatics Analysis

Our bioinformatics services offer scientists custom analysis to address real issues apart from data quality control, gene alignment, genome assembly, and functional annotations. Our bioinformatics analysis services provide solutions for both academic and industrial needs. For more details, please refer to the following table.

Table 1. Our custom bioinformatics analysis for microbial whole genome sequencing.

ANALYSIS CONTENT DETAILS
Complete genome analysis Phage genomic characteristics can be predicted to profile critical features of phages, including functional annotations.
Evolutionary genomic analysis Perform phylogenetic analyses to understand the evolutionary relationships among different phages.
Comparative genomic analysis Help scientists explore the biodiversity of phages and understanding phage-host interactions.
Functional analysis of phage interactions Interpret how phages interact with host protein from a genomics perspective.
Phage biomarker identification Use bacteriophages as biomarkers or targets to conduct disease research.
Screen for functional phages Develop phage therapy to replace antibiotics for the treatment of infectious diseases.

Sample Requirements

  • Phage Whole-Genome Sequencing 

    1. No obvious degradation, 1.8 < OD260/280 < 2.0, OD260/230 ≥ 1.8, DNA/cDNA amount ≥ 1 ug
    2. Deliverables: raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control dashboard, statistic data, and your designated bioinformatics report.
* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.



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