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Microbial Diversity in Water

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The Importance of Microbial Diversity in Water

Microbial Diversity in Water

The world’s water, such as glacier meltwater, seawater, sewage, is teeming with numerous microscopic life forms, which is second only to soil in microbial species. Microorganisms, including archaea, bacteria, fungi and protists (protozoa and algae), dominate the ecological processes of water. Some of these microbes are key participants in biogeochemical processes which are of great importance to the entire ecosystem. Some microorganisms play an important role in the processes controlling water quality, and some are crucial for the discharge of pollution into the environment. Therefore, studying the diversity and community structure of microorganisms in water is crucial for water source protection and sewage treatment through understanding biochemical evolution of microbial resources and carrying out microbial restoration in water.

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Accelerate Research and Practice in Microbial Diversity in Water

Based on our Illumina Hiseq/Miseq, Ion PGM, PacBio SMRT sequencing, and Nanopore sequencing platforms, we can not only help you identify the microorganisms presented in the water, but also analyze their diversity, abundance, and the relationship between the water environments and the microbiomes. Besides, we also provide qualitative and quantitative studies of the water microbiome base on real-time qPCR.

The Main Directions

  • Explore the differences of microbial community structure in different water areas
  • Study changes of water microbiota in diversity and abundance after certain treatment
  • Determine the degree of water pollution and remediation actions

What Can We Do?

  1. 1. Identification of water microorganisms
  2. 2. Quantitation of water microorganisms
  3. 3. Study the evolutionary relationship of water microorganisms
  4. 4. Study the functions of microorganisms in the water environments

Microbial Diversity in Water

Note: our service is for research use only.

Detectable Objects

DNA samples of water microbiome from different water sources (including river, lake, glacier meltwater, seawater, sewage, etc.) can be tested.

Detectable Microorganisms

Archaea, bacteria, fungi, protists, etc.

Technical Platforms

We are equipped with Illumina Hiseq/Miseq, Ion PGM, PacBio SMRT systems, Nanopore systems, PCR-DGGE (PCR-denaturing gradient gel electrophoresis), real-time qPCR, clone library, and other detection platforms.

Sample Requirement

    1. DNA sample: DNA ≥ 300 ng, concentration ≥ 10 ng/ul, and OD260/280 = 1.8 – 2.0.
    2. Make sure the DNA is intact and non-degraded, avoiding repeated freezing and thawing cycles.
    3. Please prepare enough dry ice or ice packs to ensure low temperature during sample delivery.

Workflow

High throughput sequencing analysis processFigure 1. High throughput sequencing analysis process

PCR-DGGE analysis processFigure 2. PCR-DGGE analysis process

Bioinformatics Analysis

Bioinformatics Analysis

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