We utilize the Oxford Nanopore technology in microbiology for several research purposes including genome sequencing, 16S rRNA sequencing, epigenomics, and transcriptomics. Our experienced and professional team executes quality management following each procedure to provide comprehensive and accurate data and data analysis.
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The Nanopore sequencing technology detects the real-time electric current of single-stranded DNA (ssDNA) as it passes through a small protein channel, called a Nanopore. The electric current data is then converted into a corresponding sequence. This technology relies on the physicochemical properties of ssDNA rather than enzymatic reactions. The sequencing speed has been greatly improved compared with next-generation sequencing systems. MinION is the only portable, real-time device for DNA and RNA sequencing in the market. The average reading length of Nanopore technology can reach tens to hundreds of Kb, and the longest reading length can reach 2 Mb. Highly contiguous genome assembly can be generated for many large and complex organisms that are deemed inaccessible to NGS methods. As a result, Nanopore sequencing could provide a more complete view of genetic content and variation.
In addition to improving read length and sequencing speed, Nanopore sequencing could reduce or eliminate sequencing bias caused by PCR amplification. The Nanopore sequencing has significantly improved de novo genome assemblies, detection of genomic structural variant, and transcriptome studies. The real-time streaming of sequence data allows for rapid insight into samples, on-demand sequencing, and dynamic workflows. Based on the superior Nanopore sequencing technology, we are dedicated to offering diverse microbial genomics services related to the following four aspects: genome analysis, 16S rRNA sequencing, epigenomics, and transcriptomics. We can better solve a series of problems like complex genome assembly, detection of structural variations and so on.
|Genome Analysis||Genome assembly||Determine the sequence of a genome using only randomly sampled sequence fragments|
|Mutation detection||Detection of SNPs, indels, and CNVs|
|Structural variation||Reveal mutational mechanisms and risk factors for chromosome rearrangement|
|Transcriptomics Analysis||Gene isoform identification||Detection, prediction, and characterization of RNA isoforms|
|Relative abundance of transcripts||Estimation and visualization of the relative abundance of mRNA transcripts|
|Identification of AS events||Identification and characterization of alternative splicing (AS) events|
|SNP calling||Detection of single nucleotide polymorphisms (SNPs)|
|Expression analysis||Detection of differentially expressed mRNA, lnRNA, circRNA, genes|
|16S rRNA Sequencing Analysis||Taxonomic assignment||OUT clustering and filtering, taxonomic assignment|
|Phylogeny analysis||Construct phylogenetic trees illustrating the evolutionary relationships among species|
|Statistical analysis||PCA, Heatmap, VENN analysis, etc.|
|Epigenomics Analysis||Data processing||Exportation of sequence data and data processing such as adapter trimming|
|Alignment and assembly||Genome assembly and polishing using NGS data|
|Methylation detection||N6-methyladenine (m6A), 5-methylcytosine (m5C), and N4-methylcytosine (m4C) motifs, etc.|
|Custom Analysis||More data mining upon your request|
Sampling kits: We provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.
Deliverables: Raw sequencing data (FASTQ), trimmed and stitched sequences, quality-control dashboard, statistic data, and your designated bioinformatics report.