Sewage sludges are the product of the biological wastewater treatment and their chemical and biological composition varies depends on the type of wastewater and pre-treatment methods. Sewage sludge may be contaminated by pathogenic microorganisms and spores, such as the potentially pathogenic bacteria (Salmonella genus), the eggs of intestinal parasites (Ascarislumbricoides, richuris sp., Toxocara sp., etc.) and coliform bacteria (Pseudomonas aeruginosa and Clostridium perfringens). So it is difficult to find a consistent way of their development. Knowledge of the physico-chemical parameters and microbiological composition of the sewage sludge is needed to determine their development. Microbial analysis of sewage sludges is very important to evaluate possible improvements in the chemical and microbiological characteristics of sewage sludges, whether there are heavy metal contents and pathogens through the composting process.Request a Quote
We can provide comprehensive microbiological testing about sewage sludges by varies technology including, high-throughput sequencing, PCR denaturing gradient gel analysis (PCR-DGGE) and real-time qPCR. According to your specific needs, we employ various sequencing platforms, including Illumina Miseq/Hiseq and PacBio Sequel instruments to obtain accurate and huge sequence data. Comprehensive bioinformatics approaches are used to investigate microbiological composition of the sewage sludge, the relationship between the physico-chemical parameters (pH, sedimentation properties, temperature, mineral substances, the dry weight, biogenic elements and heavy metals, etc.) and sewage/sludge microbiome, and the relationship between microbial populations and enzyme activities in a soil amended with sewage sludge.
Note: Our service is for research use only, and not for therapeutic or diagnostic use.
Bacteria, fungi, archaebacteria, viruses.
Next-generation sequencing (NGS), PCR denaturing gradient gel analysis (PCR-DGGE), real-time qPCR
Illumina HiSeq/MiSeq, PacBio SMRT systems, Nanopore MinION systems, PCR-DGGE, Real-time qPCR, clone library, etc.
|Fundamental Analysis||Distribution-based OTU-calling|
|Effective sequences generation|
|Rank abundance curve|
|Principal components analysis (PCA)|
|Advanced Data Analysis||LDA effectsize|
|Intermicrobial correlation analysis|