Sewage sludges are the product of the biological wastewater treatment and their chemical and biological composition varies depending on the type of wastewater and pre-treatment methods. Sewage sludge may be contaminated by pathogenic microorganisms and spores, such as the potentially pathogenic bacteria (Salmonella genus), eggs of intestinal parasites (Ascarislumbricoides, richuris sp., Toxocara sp., etc.) and coliform bacteria (Pseudomonas aeruginosa and Clostridium perfringens), so understanding their ways of development can be difficult, but achievable. To do so, knowledge such as the physico-chemical parameters and microbiological composition of the sewage sludge is needed. Analyzing microbes in sewage sludges is important, as the chemical and microbiological contents of the sludges can potentially be improved, in this case, eliminating the heavy metal contents and pathogens.Request a Quote
We can provide comprehensive microbiological testing of the sewage sludges using techniques including high-throughput sequencing, PCR denaturing gradient gel analysis (PCR-DGGE) and real-time qPCR. According to your specific needs, we employ various sequencing platforms, including Illumina Miseq/Hiseq and PacBio Sequel instruments to obtain accurate and significant amount of sequence data. Comprehensive bioinformatics approaches are used to investigate microbiological composition of the sewage sludge. We can evaluate the relationship between the physico-chemical parameters (pH, sedimentation properties, temperature, mineral substances, the dry weight, biogenic elements and heavy metals, etc.) and sewage/sludge microbiome, and the relationship between microbial populations and enzyme activities in a soil amended with sewage sludge.
Note: Our service is for research use only, and not for therapeutic or diagnostic use.
Bacteria, fungi, archaebacteria, viruses.
Next-generation sequencing (NGS), PCR denaturing gradient gel analysis (PCR-DGGE), real-time qPCR
Illumina HiSeq/MiSeq, PacBio SMRT systems, Nanopore MinION systems, PCR-DGGE, Real-time qPCR, clone library, etc.
|Fundamental Analysis||Distribution-based OTU-calling|
|Effective sequences generation|
|Rank abundance curve|
|Principal components analysis (PCA)|
|Advanced Data Analysis||LDA effectsize|
|Intermicrobial correlation analysis|