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Microbial Analysis of Sewage Sludges

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The Importance of Microbial Analysis of Sewage Sludges

The Importance of Microbial Analysis of Sewage Sludges

Sewage sludges are the product of the biological wastewater treatment and their chemical and biological composition varies depends on the type of wastewater and pre-treatment methods. Sewage sludge may be contaminated by pathogenic microorganisms and spores, such as the potentially pathogenic bacteria (Salmonella genus), the eggs of intestinal parasites (Ascarislumbricoides, richuris sp., Toxocara sp., etc.) and coliform bacteria (Pseudomonas aeruginosa and Clostridium perfringens). So it is difficult to find a consistent way of their development. Knowledge of the physico-chemical parameters and microbiological composition of the sewage sludge is needed to determine their development. Microbial analysis of sewage sludges is very important to evaluate possible improvements in the chemical and microbiological characteristics of sewage sludges, whether there are heavy metal contents and pathogens through the composting process.

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Accelerate Research and Practice in the Microbial Analysis of Sewage Sludges

We can provide comprehensive microbiological testing about sewage sludges by varies technology including, high-throughput sequencing, PCR denaturing gradient gel analysis (PCR-DGGE) and real-time qPCR. According to your specific needs, we employ various sequencing platforms, including Illumina Miseq/Hiseq and PacBio Sequel instruments to obtain accurate and huge sequence data. Comprehensive bioinformatics approaches are used to investigate microbiological composition of the sewage sludge, the relationship between the physico-chemical parameters (pH, sedimentation properties, temperature, mineral substances, the dry weight, biogenic elements and heavy metals, etc.) and sewage/sludge microbiome, and the relationship between microbial populations and enzyme activities in a soil amended with sewage sludge.

Main Research Directions

  • Understand the chemical and microbial composition of sewage sludges that determines the method of disposal.
  • The sewage sludges microbiome and sustainable agricultural development.

What Can We Do?

  1. 1. Diversity analysis of the sewage/sludge microbiome using 16S/18S/ITS sequencing or metagenomic sequencing.
  2. 2. Microbial abundance analysis using quantitative 16S/18S/ITS sequencing or real-time qPCR.
  3. 3. Compare the differences in sludge microbial communities of different wastewater treatment plants.
  4. 4. Understand the metabolic potentials of sludge microbial communities.

Note: Our service is for research use only, and not for therapeutic or diagnostic use.

Detectable Objects

Bacteria, fungi, archaebacteria, viruses.

Detection Methods

Next-generation sequencing (NGS), PCR denaturing gradient gel analysis (PCR-DGGE), real-time qPCR

Technical Platforms

Illumina HiSeq/MiSeq, PacBio SMRT systems, Nanopore MinION systems, PCR-DGGE, Real-time qPCR, clone library, etc.

Sample Requirements

    1. DNA sample: ≥ 300 ng, OD260/280 = 1.8 – 2.0, concentration ≥ 10 ng/μl.
    2. Ensure that the DNA is not degraded. Avoid repeated freezing and thawing during sample storage and shipment.
    3. Please use enough dry ice or ice packs during shipment.
    4. Nucleic acid samples should be stored at -20/-80 °C.

Bioinformatics Analysis

Fundamental Analysis Distribution-based OTU-calling
Effective sequences generation
Rarefaction curve
Shannon-Wiener curve
Rank abundance curve
Diversity index
Conventional Analysis Heatmap
VENN
Principal components analysis (PCA)
(Un)Weighted Unifrac
NMDS
Hierarchical clustering
Advanced Data Analysis LDA effectsize
Intermicrobial correlation analysis
Phylogenetic trees
Network analysis

References

  1. Błaszczyk K., Krzyśko-Łupicka T. Microbiological and physico-chemical composition of sewage derived from the food industry. Chemistry Didactics Ecology Metrology. 2013, 89-95.
  2. Leite Moretti SM, etc. Composting sewage sludge with green waste from tree pruning. Scientia Agricola. 2015, 72(5): 432-439.

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