The microecological environment in the reproductive tract plays an important role in women's health, reproductive function, and baby birth. A large number of normal microorganisms are present in the reproductive tract, adhering to the vaginal epithelial cells through the adhesion mechanism growing on the surface of the vaginal mucosa. Microbes in the reproductive tract interact with each other and are in a dynamic equilibrium state affecting not only the microbiome but also the vaginal environment. Studies have shown that reproductive tract microbiome can be affected by a variety of factors, including hormonal changes, pathogen invasion, antibiotic drugs, and behavior.Request a Quote
We can provide specific information about the diversity, abundance, evolutionary relationship of reproductive tract microbes, and the interaction among microorganisms or between the microbiome and the environment using next-generation sequencing (NGS), PacBio SMRT sequencing, or Nanopore sequencing technology coupled with powerful bioinformatics analysis. In addition, we also utilize real-time qPCR to qualitatively and quantitatively study the reproductive tract microbiome.
Note: Our services are for research use only, not for disease diagnosis and treatment.
We have successfully analyzed reproductive tract microbiome samples from human, mouse, rat, monkey, chicken, sheep, fish, horse, donkey, panda, pangolin and other animals.
Bacteria (such as lactic acid bacteria), fungi, viruses, etc.
We are equipped with High-throughput sequencing, Ion PGM, PacBio SMRT systems, Nanopore systems, real-time qPCR, PCR-DGGE, and other detection platforms.
DNA sample: total DNA ≥ 300 ng, concentration ≥ 10 ng/μL, OD260/280=1.8-2.0
Make sure the DNA is not degraded and do not freeze and thaw the DNA repeatedly.
Please prepare enough ice pack or dry ice to ensure low temperature during transportation.
Figure 1. High-throughput sequencing analysis process
Figure 2. PCR-DGGE analysis process
|OTC Generation||OTU Clustering and Species annotation|
|Relative Abundance||Veen Diagram|
|Principal Components Analysis (PCA)|
|Community Structure||Principal Coordinate Analysis (PCoA)||CCA/RDA Analysis|
|(Un)Weighted Unifrac Test|
|Structure of Microbial Colony Images|