A major threat to human health that is probable to distribute within communities is perceived as antibiotic resistance. It is accountable for rising mortality rates and poses a massive challenge to the health system. β-lactamases, enzymes that inactivate and deliver useless for the diagnosis of clinically significant Gram-negative bacterial diseases in the β-lactam family of antibiotics, bestow resistance to penicillins, cephamycins, and, in some cases, to carbapenems. For active surveillance and infection control, genetic classification of these processes of resistance is important. The appearance and qualities of specific β-lactamases perform a crucial part in the selection of suitable antibiotic therapy, as these antibiotics are often chosen to control and treat infectious diseases.
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With diverse implementations such as gene expression analysis, real-time PCR (qPCR) assays have become the instrument of preference for the fast and sensitive identification and quantification of nucleic acid in various biological specimens. For quantitative analysis of gene copy number (gene dosage) in reshaped cell lines or the appearance of mutant genes, qPCR assays are commonly seen in research laboratories. qPCR assays can be utilized in combination with reverse transcription PCR (RT-PCR) to accurately quantify modifications in gene expression such as an increase or decrease in expression in relation to changing environmental circumstances or drug treatment, by evaluating shifts in the level of cellular mRNA.
qPCR is an effective genotyping and gene expression assessment method. As of now, qPCR tests with an expanding and increasing list of targets from a greater sample size, all with more technical replicates, are becoming more and more intricate. In order to manage the transmission of bacteria, identifying resistance systems is vital and can enable identifying the best therapeutic solutions for patients. In identifying these processes, nucleic acid tests complement standard culture susceptibility testing. CD Genomics utilizes qPCR, including the extraction of DNA from bacterial cells, genetic material amplification, and successive identification using qPCR methods, for microbial antibiotic resistance gene analysis.
Our bioinformatics analysis services are flexible to your specific projects.
|Multiplex Real-time PCR||Identification of β-lactamase genes with clinical relevance|
|β-Lactamase||Identifies 9 distinct families of carbapenemases, ESBLs and plasmid-mediated ampC genes|
|ampC||Aims 6 plasmid-mediated genes of ampC resistance and can distinguish resistance of plasmid-mediated ampC β-lactamase from chromosomal resistance|
|Sequence alignment||Filtering and trimming of raw data, coverage of sequencing, sequence alignment, and genome assembly|
|Genome annotation||Annotation of open reading frames (ORFs) and analysis of comparative gene clusters using equipment such as RAST|
|ARGs detection||Disclose ARG distribution and localization, discover new ARGs; visualize ARGs|
|Comparative genomics||Uncover the molecular processes of resistant strain evolution|
Sampling kits: We provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.