Mycoplasma Detection

Service Overview

Mycoplasma exists widely in nature, and many kinds of mycoplasma can be pathogenic to humans, animals, plants and insects. Mycoplasma contamination affects metabolism, biochemical characteristics, growth status, cell structure, etc., directly impacting the productive efficiency and product quality of research laboratories, regenerative medicine, biotechnological productions and biopharmaceutical industry. Besides, mycoplasma contamination is a major problem in cell culture. We provide services to help you detect and quantitate mycoplasma in diverse samples.

Our Advantages:
  • Decades of experience in genomics service and proven expertise in microbial genomics.
  • High-resolution and high-reproducibility data.
  • Extensive experience in handling various samples.
  • Exquisite gel electropherogram.
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Introduction to our mycoplasma detection solution

Mycoplasma is a type of microorganisms that is categorized between bacteria and virus. It is the smallest and simplest prokaryote found so far. Mycoplasma lacks cytoderm, and the only organelles visible in cells are ribosomes, making it resistant to antibiotics. Mycoplasma contamination in cell cultures is a major problem in research laboratories, ranging from 5 to 35% in published reports. It severely impacts cell proliferation and culture, potentially contributing to the production of misleading research results and unqualified biotechnology products. Therefore, mycoplasma detection is important for laboratory research, medical research, drug production, etc.

We use MicroSEQ® to identify mycoplasma. The combination of the latest DNA sequencing technology and powerful analysis software can identify mycoplasma by automatic sequencing of 16S rRNA gene. Real-time PCR can further quantitate mycoplasma. We are dedicated to delivering trustworthy information on mycoplasma pollution in cell banks, raw materials, bulk and final drug products. Mycoplasma detection is also used in medical research of mycoplasma infections. We can detect about 30 different mycoplasma species, such as M. fermentans, M. hyorhinis, M. pirum, M. hominis, M. argininii, M. pneumoniae, Acholeplasma laidlawii, etc.

Project Workflow

Bioinformatics Analysis

Service Analysis content
Mycoplasma Detection Determination on whether any mycoplasma is present in your sample
Mycoplasma Identification Detection of mycoplasma species and strains
Mycoplasma Quantitation Determination of mycoplasma abundance
Custom Analysis Other analysis based on your needs

Sample Requirement

    1. DNA sample: DNA ≥ 300 ng, concentration ≥ 10 ng/ul, OD260/280 = 1.8-2.0
    2. Ensure that the DNA is not degraded and avoid repeated freezing and thawing cycles.
    3. Please use enough dry ice or ice packs during transport.

Note: Our service is for mycoplasma detection only, and not for disease diagnostic procedures.

References

  1. Young L, Sung J, Stacey G, et al. Detection of Mycoplasma in cell cultures. Nature protocols, 2010, 5(5): 929.
  2. Olarerin-George A O, Hogenesch J B. Assessing the prevalence of mycoplasma contamination in cell culture via a survey of NCBI's RNA-seq archive. Nucleic acids research, 2015, 43(5): 2535-2542.
* For research purposes only, not intended for clinical diagnosis, treatment, or individual health assessments.