SYBR Green I-based qPCR Assay for ARGs

The SYBR Green I assay is a powerful tool for detecting antibiotic resistance genes in bacteria. It is based on real-time PCR technology and uses SYBR Green I fluorescent dye to detect the presence of antibiotic resistance genes. The assay offers the possibility of simultaneous discrimination of the mutation in codon 460 and quantitative detection of the copies in mixtures. After embedding into the double-stranded DNA molecule in the PCR reaction system, an excess of SYBR fluorescent dye is added, which is specifically incorporated into the DNA double-strand and changes its conformation, absorbing 497 nm excitation light and emitting 520 nm fluorescence; whereas the dye molecule not incorporated into the DNA double-strand does not emit any fluorescence signal, thus ensuring that the increase in fluorescence signal is perfectly synchronized with the increase in the PCR product. This ensures that the increase in fluorescence signal is perfectly synchronized with the increase in the PCR product. CD Genomics offers characterization services for this assay, which can help researchers to better understand the genetic basis of antibiotic resistance.