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Oral Microbiome Research

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The Importance of Oral Microbiome

Oral Microbiome Research

The microbiome found in the mouth is highly complex with more than 700 species, including viruses, fungi, bacteria, archaea, and protozoa, some of which are primarily disease-associated. Under normal conditions, the interaction between oral microbiome, and between microbiome and host maintains a dynamic balance, but oral homeostasis is easily disturbed by external factors. The ecological imbalance of oral microorganisms can not only induce a variety of oral diseases (such as caries, periapical disease of dental pulp, periodontal disease, etc.), but are also closely related to tumor, diabetes, rheumatoid arthritis, cardiovascular disease, premature delivery and other systemic diseases, which have a great impact on human health.

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Accelerate Research and Practice in Oral Microbiome

We are equipped with Illumina Hiseq/Miseq, Ion PGM, PacBio RSII, and Nanopore MinION platforms to help you identify the microorganisms presented in the oral cavity, and analyze the diversity, abundance, evolutionary relationship, and disease implications of the oral microbiome. Besides, we also provide qualitative and quantitative studies of the oral microbiome using real-time qPCR.

The Main Directions

  • Study the differences of oral microbial in the composition and abundance between the diseased group and healthy group
  • Explore and compare the differences of oral microbial community structures among individuals at different intervention stages
  • Study changes in the oral microbiome at different developmental stages
  • Search for biomarkers or drug targets of certain diseases

What Can We Do?

  1. 1. Identification of oral microorganisms
  2. 2. Quantitation of oral microorganisms
  3. 3. Study the evolutionary relationship of oral microorganisms
  4. 4. Study the relationship between oral microorganisms and diseases

Note: Our service is for research use only, and not for therapeutic or diagnostic use.

Oral Microbiome Research

Detectable Objects

DNA samples of oral microbiome from humans and animals (such as mouse rat, sheep, monkey, horse, fish, etc.) can be tested.

Detectable Microorganisms

Viruses, fungi, bacteria, archaea, etc.

Technical Platforms

We are equipped with Illumina HiSeq/MiSeq, Ion PGM, PacBio SMRT systems, Nanopore systems, PCR-DGGE (PCR-denaturing gradient gel electrophoresis), real-time qPCR, clone library, and other detection platforms.

Sample Requirement

    1. DNA sample: DNA ≥ 500ng, concentration ≥ 10ng/ul, and OD260/280 = 1.8-2.0.
    2. Ensure that the DNA is intact and non-degraded, avoiding repeated freezing and thawing cycles.
    3. Dry ice or ice packs shall be used for sample delivery.
    4. We provide superior MicroCollect™ oral sample collection products for the collection of oral microbiome from saliva or oral cells.

Workflow

High throughput sequencing analysis process Figure 1. High throughput sequencing analysis process

PCR-DGGE analysis process Figure 2. PCR-DGGE analysis process

Bioinformatics Analysis

OTU Clustering Distribution-Based OTU-Calling
Rarefaction Curve
Shannon-Wiener Curve
Rank Abundance Curve
Diversity Index
OTU-Based Analysis Heatmap
VENN
Principal Components Analysis (PCA)
Hierarchical Clustering
Comparative Analysis RDA/CCA
PCA/PCoA
Network Analysis
Functional analysis BLASTX, KEGG, eggNOG, CAZy, CARD, ARDB etc.
PICRUSt
TAX4Fun
Phylogenetic Analysis (Un)Weighted Unifrac
Phylogenetic Trees

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