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Long-Read Metagenomic Sequencing


Long-read metagenomics sequencing is an effective, culture-free method for studies of microbial communities with considerable accuracy and reliability. We provide the long-read metagenomics sequencing service using PacBio SMRT or Nanopore instruments, which could sequence multiple organisms in parallel, and reduce assembly errors.

Our Advantages:
  • Comprehensive and flexible bioinformatics analysis content.
  • The longest reads are over 50kb, which can enhance genome assemblies.
  • The long-read technologies can span the low complexity and repetitive regions.
  • Does not rely on DNA amplification, which eliminates PCR bias.
  • Experienced and professional expert team.
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We are dedicated to providing outstanding customer service, listening to customer requests and being reachable at all times.

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Introduction to Our Long-Read Metagenomic Sequencing Platform

We provide the long-read metagenomics sequencing service based on the single-molecule sequencing technologies. Compared with metagenomic shotgun sequencing, the assembly of metagenomes is dramatically improved by long-read sequencing technologies including PacBio SMRT and nanopore sequencing. And they allow to directly detect chemical modifications without sample preprocessing for next-generation sequencing (NGS) such as bisulfite treatment of antibody pull-down. Their performance for metagenomic samples achieved >99% predicted concordance to reference sequences in soil, water, etc. Long-read metagenomic sequencing can detect very low-abundance members of the microbial communities that may be missed or are too expensive to identify for other approaches. They can identify rare species with species as low as 0.05% to comprehensively describe the composition of a microbial community and the abundance of its members.

Coupled with powerful computational algorithms and tools, long-read metagenomics technologies could provide much important information about species-level classification, microbial community structure, the abundance of microorganisms, genetic variations, gene annotation, functional annotation, pathway enrichment, microbial evolution and so on. Metagenomics enables the detection of bacteria, archaea, viruses, and eukaryotes in samples. Utilizing the long-read metagenomic sequencing can reduce assembly errors, and largely improve the resolution. Long-read metagenomics sequencing is an ideal platform for sequencing of microbial communities, particularly for the complex samples and low-diversity microbial communities.

Long-read Metagenomic Sequencing Analysis Workflow

Bioinformatics Analysis

Our bioinformatics analysis includes five parts: quality control, genome assembly, taxonomic assignment, functional annotation, visualization analysis. We are flexible to your needs.

Bioinformatics Analysis Details
Quality Control Filtering and trimming of raw data
Genome Assembly Reconstruct the genome
Taxonomic Assignment MIRA 4.0, WIMP, Kraken, One Codex, etc.
Functional Annotation KEGG Pathway analysis
eggNOG Evolutionary genealogy of genes: Non-supervised Orthologous Groups
CAZy Carbohydrate-Active enZYmes analysis
CARD Analysis of genes associating with drug resistance
Visualization Analysis PCA Visualization of metagenomics data
VENN
Heatmap
Custom Analysis More bioinformatics analysis are accessible according to your needs

Sample Requirement

Sampling kits: We provide a complete range of microbial sampling kits for clients, including microbial collection products, DNA/RNA isolation kits, and accessories for storage and mailing.

Deliverables: raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control report result, statistic data, and your designated bioinformatics report, visualized pictures.

Solutions

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